SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

Ch. 7 - Taking a Picture3. The Get Bias Frame window will direct you to “Block all light to the camera”.4. Cover the optical train or find another method to block light to the camera’s CCD.Click on OK.The program will acquire the number of short dark frame exposures required to calculate the BiasFrame.5. A window will appear asking you to save the bias framedata to a file for use in a specific image setup. Input theinformation as required.6. Move the specimen back into the field of view, andperform the image capture.Get Thermal Frame (Dark Noise Subtraction)Get Thermal Frame is a function that allows you to collect data to minimize the effect of thermal noise inyour captured image. For a more detailed discussion of the theory and benefits of the Dark NoiseSubtraction process, refer to Chapter 5, Preparing to Take a Picture.To use Thermal Frame Subtraction follow these steps:1. From the Camera menu, select Get Thermal Frame. The Get Thermal Frame dialog boxappears.2. The program will ask for the number of imageframes to capture to determine the Thermal Framereference noise level. You may select a numberfrom 1-50 frames. The more frames you choose,the more accurate the result should be, however,the process will take longer.3. The Get Thermal Frame window will direct you to “Move to Background”. Do so and thenclick on Begin. The program will acquire the number of exposures required to calculate theThermal Frame.4. The Get Thermal Frame window will direct you to “Block all light from the camera”5. Cover the optical train or find another method to block light to the camera’s CCD.6. Click on OK. The program will acquire the number of exposures required to calculate theThermal Frame.7. A window will appear asking you to save the Thermal frame data to a file for use in a specificimage setup. Input the information as required.8. Move the specimen back into the field of view, and perform the image capture.User Guide to the SPOT Insight Camera 131