SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

Ch. 7 - Taking a PictureRecall Previous ExposureRecall Previous Exposure assigns the exposure time and gain from your last image capture to an imagesetup. The Recall Previous Exposure feature is available only after you have taken a picture.To recall previous exposure values, follow these steps:1. Capture an image.2. From the Camera menu, select Recall Previous Exposure. The Exposure window displays the valuefrom the last image capture (see above).Note: If you change the current image setup, the Recall Previous Exposure option will beunavailable until you take another picture.3. Select an image setup from the drop-down box.4. Click OK.5. Use Get Image (exposure from setup) to capture an image with the new exposure and gain values.Compute White Balance Values (Insight Color Cameras)White balance is the ratio of red, green, and blue exposure times necessary to achieve the proper colorrendition for an image. Many factors impact the coloration of an image capture, including the lampvoltage, the coloration of the glass in the slide, and the coloration of the lenses in the microscope’sobjectives. To minimize the impact of these factors, use the Compute White Balance feature. When youperform a white balance calculation, SPOT samples the color of the light from your light source after it haspassed through the slide and the objective, and then calculates the exposure values needed to produceimages where the white areas are white and the color areas are true.You should perform a new white balance calculation when you:• Start a new image capture session.• Change the lamp voltage.• Switch to a different objective on the microscope.When you open an image setup, the Image Setup screen displays the default white balance values for thatSetup, which are the values that were last saved to the Image Setup. If you capture an image withoutcomputing a new white balance, SPOT uses these default values. Refer to Ch. 5 – Preparing to Take aPicture for a step-by-step description of how to compute a white balance.Get Bias Frame (Bias Frame Subtraction) (Insight Monochromeand 3-shot in Monochrome mode)Get Bias Frame allows you to collect data used to reduce the effect of noise in your captured image,specifically noise related to the bias (offset) used to adjust for the readout electronics. For a more detaileddiscussion of Bias Frame Subtractions, refer to Chapter 5, Preparing to Take a Picture.To use Bias Frame Subtraction follow these steps:1. From the Camera menu, select Get Bias Frame. The Get Bias Framedialog box appears.2. The program will ask for the number of image frames to capture todetermine the Bias Frame reference noise level. You may select anumber from 1-50 frames. The more frames you choose, the moreaccurate the result should be, however, the process will take a longer time.130 User Guide to the SPOT Insight Camera