SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

Ch. 14 – Using Other Windows Imaging Applications in Advanced Modeacquired.Make Shutter SoundThe user may select whether to utilize the audible shutter sound when capturing an image.DeviceThe Device option allows you to select from a variety of image acquisition devices for previewing andcapturing images through the TWAIN interface. These can be selected and changed “on the fly”. Onceyou have selected a different device, the TWAIN window will update automatically to include optionsconsistent with the selected device.The Palettes option allows Palettes (Insight B/W)The Palettes option allows you to define new palettes and edit existing palettes.SPOT color palettes allow you to apply specific hue and saturation values to monochrome (8 bit or 12 bit)images. Palettes are useful in fluorescence applications where a sample is fluorescing at a singlewavelength, and you want the acquired image to have the correct color. For such a case, you could create apalette to represent the color of the fluorescent dye and then apply that palette to the captured image.There are two ways to apply a palette to an image:• You can associate a palette with an Image Setup, as discussed in Ch. 5 - Preparing to Take a Picture.• You can apply a palette to a captured image by selecting the Set Palette option from the Edit menu.Refer to Ch. 8 – Viewing, Editing, and Annotating Images for more details.To define a new palette,follow these steps:1. From the Setupmenu, selectPalettes.2. Click on Add. TheColor Palette dialogappears as shownhere:3. Use the Hue andSaturation selectionlists, select aWavelength, ormove the cross hairson the color wheel to enter the values.− Wavelength allows the user to specify the exact wavelength ranging in value from 400nm to 700nm. Wavelengths will automatically correspond to various Hues at full (100%) saturation.−−Hue is the designation for the actual color, as distinguished from others in the color spectrum.Hue is derived from the color wheel and is expressed in the color's angular location (in degrees) onthe wheel. (e.g., 0° to 360°).Saturation is the proportion of perceived pure hue in the color. Saturation (in the SPOT software)is measured on a numerical scale of 0 to 100, where zero is pure gray and 100 is pure hue (e.g.,day glow).4. Click on OK.User Guide to the SPOT Insight Camera 271