SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

Taking Your First PictureQuick Start4. Perform a White Balance on the image.a) Click on the White Balance toolbar button. The liveimage display pauses, and the Compute WhiteBalance dialog prompts you with a number ofoptions to calibrate the white balance.b) Follow one of the instructions, based upon yourapplication.c) Click on Begin. The Compute White Balance dialogdisplays Computing Exposure, and the LiveImage window restarts and computes the newexposure settings.5. Click on the Image Settings toolbar button.The Image Settings window appears. Ensure that thesettings are as follows:Main Window:• Profile – Default• Exposure - Auto• Image Type – (your type of microscopy)• Brightness – 1.00• Gamma – 1.00• Quality/Speed – Quality (left-most position)• Chip Imaging Area – Full Chip• Live Image Auto Brightness - Selected• Flatfield – unselected• Correct Color – selectedMore Image Settings:• Image Flip – as needed• Rotate 90° – as needed• Minimum Exposure – unselected• Live Image Maximum – default (10 sec)• Noise Filter – selectedThe suggested settings are designed to give you qualityimage captures in most, but not all cases. If your workinvolves fluorescence techniques, for example, you willneed to adjust the Image Type, brightness, gamma andQuality/Speed settings accordingly. For more details onselecting Image Settings, refer to Ch. 3 – The BasicProgram Mode.6. Click on the Get Image toolbar button.The Get Image dialog box displays the status of thecapture as it is downloaded to your screen.Because Get Image uses the values from the live imageview, the image capture skips the initial exposurecomputation sequence.20 User Guide to the SPOT Insight Camera