SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

Ch. 4 – Advanced Program ModeSetupThe Setup menu options allow you to define operational standards used for image capture and editing.Refer to the sections below for descriptions of each option.Image SetupsIn the SPOT software, an image setup serves two purposes:• It defines the way a picture will be taken.• It defines the way the program will automatically modify a picture after it is taken.Pre-configured image setups for standard microscope techniques (e.g., brightfield, fluorescence, etc.) helpnovice users to take good pictures the first time. Experienced users can modify these pre-configured setupsas needed. Refer to Ch. 5 - Preparing to Take a Picture for details.Calibration SetupsWith the SPOT program, you can use a stage micrometer tocalibrate each objective on your microscope. Once calibrated,you can use the SPOT program to perform basic measurementson an image. Refer to Ch. 8, Viewing, Editing, and AnnotatingImages for more information on using the measurementfunctions.To calibrate an objective, follow these steps:1. Place the stage micrometer under the objective.2. Adjust the microscope to display as much of the stage micrometer as possible. You can use the LiveImage option, as described in Ch. 6 – Using Live Images, to help position the micrometer3. Focus on it. Use the Live Image option.4. Capture an image of the stage micrometer. Refer to Ch. 7 – Taking a Picture for details.5. From the Setup menu, select Calibration Setups. The CalibrationSetups window appears (see right).6. Click on Add. The Calibration Setup window appears.7. Enter the name of thecalibration setup. Typicallythis corresponds to themagnification of theobjective (e.g., 4x).8. Using the mouse, draw thecalibration line along thelength of the stagemicrometer image. Whenyou draw the line, keep these guidelines in mind:−−−Keep the line as straight as possible.Make the line as long as possible.Use the largest unit of measurement that the micrometer allows for.70 User Guide to the SPOT Insight Camera