SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

Ch. 5 – Preparing to Take a PicturePalette (B/W; 3-Shot)SPOT color palettes allow you to apply specific hue and saturation values to monochrome (8 bit or 12 bit)images. Palettes are useful in fluorescence applications where a sample is fluorescing at a singlewavelength, and you want the acquired image to have the correct color. For such a case, you could create apalette to represent the color of the fluorescent dye and then apply that palette to the captured image. ThePalette option on the Image Setup window allows you to automatically apply a palette to a monochromeimage capture.Note: Applying a color palette to an image capture does not affect image data.To apply a palette, select one from the drop down list. There are four pre-defined palettes: Red, Green,Blue, and Grayscale. Because the Palettes option applies only to monochrome images, it is available onlywhen either an 8 bpp or 12 bpp Pixel Bit Depth is selected. You can define custom palettes using thePalettes option on the Setup men. Refer to Ch. 4 – The Advanced Program Mode for more information.Exposure WindowThe Exposure window selections tell the camera what settings to use when taking a picture. Carefullyconsidered definition of these settings helps to ensure that your images come out as planned. The Exposureoptions vary according to two variables:• The Insight camera model that you have – Color, B/W, or 3-Shot.• The Bits per pixel settingEach Exposure option is described below. If an option is unique to a specific camera, this is indicatedparenthetically.Note: Please note that this window is where you select between allowing the program todetermine the exposure automatically (Auto-exposure) or setting it yourself (Manual). Thesubwindows of Auto-Exposure and Manual Exposure are where you set the details of eitherprotocol.92 User Guide to the SPOT Insight Camera