SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

Ch. 6 – Using Live ImagesControlsFit to WindowReset AreaTransferSnapFaster ReadoutOpen the Live Image Controls dialog box (see below). From this dialog box, you can define theviewing/capture area, and adjust gamma, brightness level, and dim image quality. You can alsoelect to display either the full chip or a region of interest. Refer to the steps below for moredetails on the Live Image Controls window.Size images to automatically fit the resizable display window.Return to the non-zoomed view of the live image.Transfer the live image directly to the SPOT viewing window, where it can be edited and/orsaved to file. The Transfer button is inactive until you Pause the real-time view. The Transferoption is limited in that all images are 8 bits per channel (i.e., because they are from live mode).The Transfer option should not be used for image captures that require highly accuratebrightness measurements.Capture the live image using the settings from the Live Image Controls dialog. When you clickon the Snap button, the Get Image status dialogs appear, just as they do when you select GetImage (exposure from setup) from the Camera menu. When you use the Snap option tocapture an image, you avoid the exposure computation sequence that typically precedes imagecaptures.Faster Readout enables you to “speed up” the live image display (higher frame rates) in caseswhere you are willing to sacrifice some resolution for a higher frame rate.Live Images are Different Than Captured ImagesWhen you view a live image, keep in mind that the captured version of the image will not be identical tothe live image view. This is due to two factors:• Live images are raw pixel data. Captured images are digitally processed to give a more accurate colorrendition. For individual image captures, there is enough time to process the data. For each image inthe live image stream, there is not enough time to process the data.• Live Image gamma adjustments are made in the RGB color space. Captured image gammaadjustments are made in the HSL color space (HSV for fluorescence). This is because gammaadjustments can be made quickly in the RGB color space, but are very slow in the HSV and HSL colorspaces. Refer to Ch. 5 – Preparing to Take a Picture for more information on gamma adjustment andcolor spaces.Note: HSL and HSV gamma adjustment for captured images applies only to images captured usingthe Get Image (exposure from Setup) option. Gamma adjustment for images captured using the Snaplive image option is always made in the RGB color space.Live Image ControlsTo adjust the live image view settings, click on the Controls button at the bottom of the Live Imagewindow. The Live Image Controls dialog appears. Depending upon whether your image settings forexposure (Chapter 5) are set to Auto or Manual, one of two windows will appear, each slightly different.Each window, however, will have a common section including the following options.Filter Color (Insight 3-Shot)Your selection should provide the highest level of contrast for theimage. Because different colors provide different levels of contrast,the best way to determine a color is to preview it by clicking on thecolor and seeing how it affects the view of the specimen.• RGB – View a color preview of your specimen116 User Guide to the SPOT Insight Camera