SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

Ch. 17 – Using Other Mac Imaging Application in Basic ModeExposure: Auto or ManualThe Exposure option gives you the choice of having the camera calculate exposure time (Auto), or settingthe exposure on your own (Manual). The exposure options will vary according to whether you selectAuto or Manual, as illustrated below.AutoManualWhen you enter one of the above exposure settings, note that it applies both to the live image that you areviewing and the captured image.Auto Exposure optionsImage TypeAn Image Type tells the SPOT program how to expose and process image captures. Each of the 11available Image Types (see below) corresponds to a microscopy technique: select the Image Type thatmatches the technique you are using. The camera will capture the image accordingly. The table belowdescribes typical situations for each image type.Image TypeBrightfield-transmitted lightDarkfield-transmitted lightFluorescenceNomarski DIC/HoffmanPhase contrastPolarized lightBrightfield-reflected lightDarkfield-reflected lightDescriptionStained specimens on glass slides using transmitted light microscopes.Bright specimens on dark backgrounds using transmitted light darkfield technique.Fluorescence specimens not fluorescing in a single red, green, or blue primary colorSpecimens being viewed with either Nomarski DIC or Hoffman techniques.Specimens being viewed using the phase contrast technique.Specimens being viewed with polarized light techniques.Polished metallurgical specimens and semi-conductors using a microscope with avertical illuminator.Bright areas of specimens being viewed with darkfield, reflected light techniques.304 User Guide to the SPOT Insight Camera