SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

Ch. 3 – Basic Program ModeBrightfield-reflected lightBrightfield-transmitted lightDarkfield-reflected lightDarkfield-transmitted lightFluorescenceNomarski DIC/HoffmanPhase contrastPolarized lightStereo/macro-metallic glintsStereo/macro–no glintsStereo/macro–shiny glintsPolished metallurgical specimens and semi-conductors using a microscope with avertical illuminator.Stained specimens on glass slides using transmitted light microscopes.Bright areas of specimens being viewed with darkfield, reflected light techniques.Bright specimens on dark backgrounds using transmitted light darkfield technique.Fluorescence specimens not fluorescing in a single red, green, or blue primary colorSpecimens being viewed with either Nomarski DIC or Hoffman techniques.Specimens being viewed using the phase contrast technique.Specimens being viewed with polarized light techniques.Metallic specimens with intense glints.Matte specimens without bright glints.Shiny specimens with bright glints.BrightnessEnter a Brightness value between .24 and 34.A brightness value of 1, the default value, gives you the correct image brightness for most images. TheBrightness control allows you to overexpose or underexpose (darken or lighten) the image area. In manycases, an image has varying levels of brightness; to see a specific area better you might want to lighten ordarken the image. When using the Brightness control, the SPOT camera uses an algorithm that combinesgain and exposure time to provide the fastest possible image update rate (shutter speed) in live mode:• When overexposing images to make them brighter (i.e., entering values greater than one), the camerafirst selects the highest possible gain before lengthening the exposure time. Because gain alsobrightens an image, less exposure time is needed.• When underexposing images to make them darker (i.e., entering values less then one) the camera usesthe lowest possible exposure time before lowering the gain.User Guide to the SPOT Insight Camera 35