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SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

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Ch. 5 – Preparing to Take a PictureTypeAutoWhen you select Auto, the camera samples light levels in the image plane (as the initial part of the imagecapture) <strong>and</strong> uses the values to calculate the final exposure time.<strong>Manual</strong>When you select <strong>Manual</strong>, you specify the exposure times. This can be done either by entering a value orvalues, using the Compute Exposure option, or by the Recall Previous Exposure option.Use: Red Green Blue (3-Shot; 24 <strong>and</strong> 36 bit RGB)Use: Red Green Blue specifies the colors that will be exposed. For most situations, you should select allthree colors. However, in situations where you know that your specimen lacks one or two of the colors,you can avoid the additional exposure <strong>and</strong> download time by “turning off” (deselecting) the missing colors.For example, if you have a pumpkin colored fluorescence sample with no blue in it, you can deselect theblue value. The camera only exposes the red <strong>and</strong> green values.Note: When you add a new 24 bpp or 36 bpp image setup, all three colors are selected as thedefault.To set the color filters <strong>for</strong> capturing a color (RGB) image, follow these steps:1. Ensure that the Pixel Bit Depth is set to either 24 (color) or 36 (color).2. Click on either the Auto-Exposure or the User-Defined Exposure option.3. Deselect one or two of the color option boxes, or leave all three checked.− Red− Green− BlueTo save the image setup, at least one color must be selected.To recall the exposure time <strong>and</strong> gain from the previously captured image, follow these steps:1. From the Camera menu, select the Recall PreviousExposure option. The Exposure window displays theexposure time <strong>and</strong> gain used <strong>for</strong> the previously capturedimage2. Select the setup that you want to save the exposure settingsto, <strong>and</strong> click on OK. The settings are saved to the imagesetup that you specify.Note: When you save the previously usedexposure value to an image setup, itautomatically changes the setup from Auto –Exposure to User Defined.User Guide to the <strong>SPOT</strong> <strong>Insight</strong> Camera 93

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