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SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

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Ch. 8 - Viewing, Editing, <strong>and</strong> Annotating ImagesDuplicate ImageClick on the Duplicate Image option to create a copy of the active (on-screen) image.Annotation – Calibration <strong>and</strong> Measurement MarksThe Add Calibration Mark <strong>and</strong> Add Measurement options enableyou to add calibration <strong>and</strong> measurement references to <strong>SPOT</strong>images.Add Calibration MarkThe Add Calibration Mark feature allows you to insert a calibration mark into animage capture. To add a calibration mark, follow these steps:1. If you have not already calibrated the objectives on your microscope, do so.Refer to Ch. 4 – The <strong>Advanced</strong> Program Mode <strong>for</strong> details.2. From the Edit menu, select Add Calibration Mark. The Calibration Markwindow appears.3. Select a Calibration Setup (e.g., 15x) <strong>for</strong> the objective that you are using.4. Move the cursor to the point in the image where you want to insert thecalibration mark <strong>and</strong> click at that point. The calibration mark appears with thedefault appearance settings.Note: If you are not satisfied with theplacement of the calibration mark, you canclick on <strong>and</strong> drag the mark to a new position.Clicking on <strong>and</strong> dragging the bar moves boththe bar <strong>and</strong> the number. Clicking on <strong>and</strong>dragging the number moves only the number.5. Using the on-screenmark as a reference,modify theAppearance options:a) Orientation –Check theappropriatebox to select either a horizontal or vertical display <strong>for</strong> the calibrationmark.b) Line Thickness – Select a line thickness between 1 (thinnest) <strong>and</strong> 5 (thickest).c) Show Text – Select this checkbox if you would like the calibration measurement text to beshown along with the calibration line.d) Decimals – Select the number of decimals that appear in the calibration mark number. Choosefrom a range of zero decimals (1) to five decimals (1.00000)e) Color – Click on the Color button to define the color of the calibration mark. You can eitherchoose from a palette of basic colors or define a custom color to provide the best display.f) Font – Define the font, point size, <strong>and</strong> appearance.User Guide to the <strong>SPOT</strong> <strong>Insight</strong> Camera 161

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