SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

Ch. 5 – Preparing to Take a PictureExternal Shutter LagWhen using an external shutter - to shutter a fluorescence illuminator, for example - there is a delaybetween the application of voltage andthe shutter opening to 100 %, as is thecase with any mechanical shutter (seethe graph at right). This delaybetween the application of voltage andthe full opening of the shutter isreferred to as the external shutter lag.SPOT Insight cameras featureelectronic shutters that open almostinstantaneously. When using anexternal shutter with a SPOT Insightcamera, then, the SPOT shutter needsto be delayed until the mechanicalshutter is fully open. To avoidtriggering the electronic shutter beforethe external shutter has had a chanceto open fully, check the External Shutter Lag box,Figure 5- 2: External Shutter Lag Timeand enter the external shutter lag time value in milliseconds. To determine the correct value, refer to thespecifications guide that accompanies the external shutter.Auto-Exposure WindowThe Auto-exposure window indicates and allows the user to modify further settings that will be used whenauto-exposure imaging is selected in the Exposure window.Image TypeWhen using Auto-Exposure, the SPOT software provides 11 image type settings that correspond to varioustypes of microscopy. The image type that you enter when creating a new setup appears on the Image Setupwindow. You can enter a new Image Type from the Image Setup window. Refer to the Accessing theImage Setup Window section of this chapter for descriptions of the different image types.User Guide to the SPOT Insight Camera 95