SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

Ch. 3 – Basic Program ModePerform the image capture. SPOT automatically uses the flatfield image to correct the capture.Correct Color TechnologyThe Correct Color option utilizes camera dependent color correction profiles that optimize the matching ofcolors between your microscope specimen, the live image you view on the screen of your monitor, and theimage you capture with your SPOT camera. This is done by transforming the raw camera color dataallowing it to be adjusted to display the closest approximation of the same color on your monitor.Correct Color Technology has been developed based on color profiling specifications develop by the ICC(International Color Consortium®).To use the Correct Color feature in Advanced Mode, simply make sure the checkbox is checked in theimage setup window (under post processing) and then in the live image controls. In Basic Mode, simplycheck the checkbox in the image settings window. In TWAIN or Mac 3 rd party mode, check the checkboxfor Correct Color.In order to get the best results using this feature, you should make sure you set up your imaging systemincluding your computer monitor appropriately. See Appendix F for more information on system set-up.Be aware that the color correction transformations that occur when you use Correct Color Technology willbe applied to the raw image data, leaving a modified image file. If you will require the raw data for furtheranalysis of your image, you should uncheck the Correct Color checkbox.More (Additional Orientation and Exposure Settings)When you click on More, the program displays a dialog ofadditional orientation and exposure settings, as shown at right:FlipSpecify whether images will be flipped during the image capture/live image viewing process.• Select Horizontal to flip the image from left to right.• Select Vertical to flip the image from top to bottom.• Select both options to flip the image from left to right and from top to bottom.Rotate 90°Note: The screen capture at right is from anInsight 3-Shot – the Filter Color, Binning, andDisplay Images in Grayscale options do notappear with other Insight models.Specify whether images will be rotated during theimage capture/live image viewing process.• Select None if you do not want the image to berotated.• Select Right to rotate the image clockwise 90degrees.• Select Left to rotate the image counterclockwise90 degrees.Exposure Limits - MinimumBecause SPOT Insight cameras are capable ofextremely short exposure times, light sources withFigure 3- 3: Setting a Minimum Exposure toAccommodate Varied Light Sources (Basic Mode)User Guide to the SPOT Insight Camera 39