SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

Ch. 17 – Using Other Mac Imaging Application in Basic ModeUsing the Live Image to Define Image Capture SettingsWhen you open the Basic mode 3rd Party Interface, the Live Image window is, by default, running, asindicated by the Live Mode Running message in the status bar. The settings options in the 3rd Partyinterface window enable you to optimize the live image view, and then capture an image using thesesettings. In the 3rd Party Interface, you can:• Create profiles to use as pre-defined image capture settings.• Modify exposure settings• Define a specific portion of the chip to be used in the image capture.• Modify the screen orientation of the specimen.The following sections describe all of the Live Image/Image Capture Settings.ProfileThe Profile option allows you to create and re-use sets of live image/image capture settings. When youopen the 3rd Party interface, the Default Profile is selected. When you make changes to any of the liveimage/image capture settings, these changes can be saved to a Profile – either the Default or one that youhave defined. For example, if you gamma adjust a live image and then decide that the result is ideal for thetype of specimen you are working with, you could create a new Profile with the gamma and exposurevalues and name it for that type of specimen – i.e., “Diseased Cells.”When you close the SPOT Camera window, the program prompts you to save changes. Carefullyconsider the effect of your choice:• Clicking Yes modifies the Profile according to the adjustments that you made. Future image capturestaken with that Profile will reflect these changes. For example, if you rotated a live image, and thenclicked Yes after closing the window, all future captures taken with that Profile would be rotated in thesame way.• Clicking No retains the original values of the Profile. This allows you to maintain a unique set ofimage view and capture settings that can be re-applied to future captures of similar specimens.To add, edit, or delete Profiles, right-click on the Profile box. Right clicking gives the following options:• Add – Add a new Profile. When you add a new Profile, the program prompts you to name it.• Save – Save a Profile that you have modified.• Rename – Rename an existing Profile.• Delete - Delete a Profile. This option appears only after you have defined a second Profile in additionto the Default Profile.User Guide to the SPOT Insight Camera 303