SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

Ch. 17 – Using Other Mac Imaging Application in Basic ModeRotate 90°Specify whether images will be rotated in the live image view and during the image capture process.• Select None if you do not want the image to be rotated.• Select Right to rotate the image clockwise 90 degrees.• Select Left to rotate the image counterclockwise 90 degrees.Exposure Limits –MinimumBecause SPOT Insight cameras arecapable of extremely short exposuretimes, light sources with intensitiesthat vary with the waveform of the60 Hz line supply (e.g., fluorescentlamps, 120 volt incandescent bulbs,and 6 volt 20 watt halogen lamps)can cause the Live Image window toflicker and result in incorrectbrightness and color balancereadings in captured images. Thisoption allows you to correct forthese problems by limiting thecamera to a minimum exposure timethat is greater than the pulse intervalof the light source. Consider theillustration to the right. In this case, the pulse interval of the fluorescent light source is 8.3 ms. Entering aMinimum Exposure of 25 ms ensures that the camera’s exposure time is greater than the pulse interval ofthe light source and that any error isminimized. The greater the value above 25Setting a Minimum Exposure toms, the more the error is reduced.Accommodate Varied Light SourcesAlternatively, you can enter any multiple of8.333 ms. To enter a minimum exposure, check the Minimum box, and enter a value in milliseconds.Exposure Limits – Live Image MaximumBecause The Live Image Maximum option helps you to avoid unnecessarily long exposures when viewinga live image. Consider the following situation: When you change from a lower resolution objective to ahigher resolution objective, the camera detects a brief interval of blackness. When the Auto-Brightnessoption (see above) is enabled, it compensates by modifying the exposure time to maintain a constantbrightness level. This results in you having to wait for the camera to compute a lengthy and incorrectexposure for the black interval. Using the Live Image Maximum option, you can limit this exposure time.For most cases, Diagnostic Instruments recommends a value of 0.5 seconds.Similarly, the Live Image Maximum option can also be used in cases where the microscope’s light pathselector is incorrectly set.Filter Color (Insight 3-Shot)The Filter Color option specifies the color or colors that will be exposed in the live image preview/imagecapture.• Red – Preview/capture a monochrome preview of your specimen using the red state of the LCD filter.• Green – Preview/capture a monochrome preview of your specimen using the green state of the LCDfilter.User Guide to the SPOT Insight Camera 311