12.07.2015 Views

SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

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Ch. 5 – Preparing to Take a Picture3. From the Camera menu, select the Compute White Balance Values option, or use the toolbar <strong>and</strong> clickon the Compute White Balance Values button. The Compute White Balance window appears.4. Click on Begin to start the calculation. The message Computing white balance valuesdisplays, as the camera samples the light. When thecamera finishes calculating the white balance values,<strong>SPOT</strong> displays the computed white balance values.Note: Due to the inherently poor bluelight sensitivity of CCD chips <strong>and</strong> theenergy distribution of halogen bulbs, theblue value is often much higher than thered <strong>and</strong> green values.5. To change the setup that the new white balance valuesare saved to, select a setup from the drop - down box.6. Click on OK. The program returns to the main editing<strong>and</strong> viewing window.7. To edit the white balance values that you saved, open the image setup (i.e., Brightfield from theprevious example). The values should appear in the Red, Green, <strong>and</strong> Blue selection bars. Edit thevalues as needed.Spot Metering AreaSimilar to the way photographers spot meter their cameras on a particular location within their subjectimage plane, the <strong>SPOT</strong> user can do the same within the microscope’s imaging plane. The spot meteringfeature allows the user to best select an area of the image to be used as the region of interest <strong>for</strong> theautomatic exposure level determined by the software.Users may select any rectangular image area less than or equal to the full image area. This can beaccomplished in one of two ways. The user may drag an area of interest on the captured window screen.This automatically will insert the four side pixel values in the spot metering area of the image setupwindow. Alternatively, the user can input the four side values directly.For a further discussion of the step by step details of these procedures, please refer to Chapter 7.<strong>Manual</strong> Exposure WindowWhen you select <strong>Manual</strong>Exposure, the followingWindow options areavailable.External Trigger<strong>SPOT</strong> gives you threeoptions in the manualexposure window <strong>for</strong>setting an externaltrigger. The defaultoption is None alongwith options <strong>for</strong> Edge<strong>and</strong> Bulb triggers.User Guide to the <strong>SPOT</strong> <strong>Insight</strong> Camera 99

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