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SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

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Ch. 17 – Using Other Mac Imaging Application in <strong>Basic</strong> ModeCorrect Color TechnologyThe Correct Color option utilizes camera dependent color correction profiles that optimize the matching ofcolors between your microscope specimen, the live image you view on the screen of your monitor, <strong>and</strong> theimage you capture with your <strong>SPOT</strong> camera. This is done by trans<strong>for</strong>ming the raw camera color dataallowing it to be adjusted to display the closest approximation of the same color on your monitor.Correct Color Technology has been developed based on color profiling specifications develop by the ICC(International Color Consortium®).In order to get the best results using this feature, you should make sure you set up your imaging systemincluding your computer monitor appropriately. See Appendix F <strong>for</strong> more in<strong>for</strong>mation on system set-up.Be aware that the color correction trans<strong>for</strong>mations that occur when you use Correct Color Technology willbe applied to the raw image data, leaving a modified image file. If you will require the raw data <strong>for</strong> furtheranalysis of your image, you should uncheck the Correct Color checkbox.Fit to WindowIn addition to automatically resizing images to fit within the live image window, the Fit to Window optionalso determines cursor behavior in the live image view.• When the Fit to Window option is checked, the live image window cursor becomes a zoom tool. Tozoom in on a portion of a live image, click <strong>and</strong> drag over the area that you want to zoom in on. Toreturn to the non-zoomed image, click on the Full Chip option.• When the Fit to Window option is not checked, the cursor is a cropping tool. If you select a portion ofan image, the program displays only the selected area <strong>and</strong> discards the rest of the image. To return tothe non-cropped image, click on the Full Chip option.These points apply only when the Always Show Full Chip in Live Mode (see above) is not selected. Formore details, refer back to the Chip Imaging Area section (see above).More (Additional Orientation <strong>and</strong> Exposure Settings)When you click on More, the programdisplays a dialog of additionalorientation <strong>and</strong> exposure settings <strong>for</strong>the 3rd Party Interface, as shown atright.<strong>Insight</strong>3-Shot<strong>Insight</strong> 3-Shot users willalso see the Filter Color,Binning, <strong>and</strong> DisplayImages in Grayscaleoptions (see below).FlipSpecify whether images will be flipped in the live image view <strong>and</strong> during the image capture process.• Select Horizontal to flip the image from left to right.• Select Vertical to flip the image from top to bottom.• Select both options to flip the image from left to right <strong>and</strong> from top to bottom.310 User Guide to the <strong>SPOT</strong> <strong>Insight</strong> Camera

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