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SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

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Ch. 17 – Using Other Mac Imaging Application in <strong>Basic</strong> Mode• Blue - Preview/capture a monochrome preview of your specimen using the blue state of the LCD filter.• Clear – Preview/capture a monochrome preview of your specimen using the clear state of the LCDfilter.Display Images in Grayscale (<strong>Insight</strong> 3-Shot)To counter the difficulty of viewing, focusing, <strong>and</strong> adjusting monochrome images in live mode, theDisplay Images in Grayscale option enables you to view a monochrome image in grayscale, as opposed towith the red, green, or blue filter. This option applies only when the Filter Color (see above) is set to Red,Green, or Blue.Binning (<strong>Insight</strong> 3-Shot)Binning combines adjacent pixels on the CCD chip, thereby reducing the number of pixels used in theimage capture. Binning is useful when you have dim images <strong>and</strong> want to increase sensitivity <strong>and</strong> reducedownload time. This gives you higher frame rates <strong>and</strong> less noisy images. But, as binning level increases,image resolution decreases. All of these factors should be considered when you select a binning option.There are four binning options:• None 1 pixel = 1 pixel• 2 x 2 4 pixels = 1 pixel• 3 x 3 9 pixels = 1 pixel• 4 x 4 16 pixels = 1 pixelNoise FilterThe Noise Filter corrects <strong>for</strong> the effects of electrical or thermal noise. When capturing dim images withlengthy exposure times, the noise filter acts as a “virtual cooler” <strong>for</strong> the <strong>Insight</strong> camera, eliminating “hotpixels.”The Noise Filter checks each pixel on the CCD chip as described below:1. The program compares the value of each red pixel with the values of the eight neighboring red pixels.2. If none of the eight neighboring red pixels has a value within ±15 % (i.e., the threshold value that the<strong>SPOT</strong> program uses) of the value <strong>for</strong> the red pixel being checked, the value <strong>for</strong> the red pixel beingchecked is replaced with the average value of the eight neighboring red pixels.3. The program repeats this procedure <strong>for</strong> the values of the green <strong>and</strong> blue pixels being checked.To activate the Noise Filter, check the Noise Filter box. The program runs the noise filter each time animage is captured using the Preview or Acquire options.Taking a PictureFrom the <strong>SPOT</strong> 3rd Party Interface, you can capture images in different ways:• Preview - You can capture a Preview image. Preview images remain available in the 3rd Partywindow <strong>for</strong> basic editing <strong>and</strong> serve as the basis <strong>for</strong> the final image capture. When you click on thePreview button, the Get Image dialog displays the capture status as it is captured, <strong>and</strong> the capturedimage then replaces in the Live Image in the window. Note that the status bar indicated that you areviewing a Preview image.• Transfer - If you like the Preview image, you can Transfer it image directly to the third partyapplication that you are using. The Transfer button is active only <strong>for</strong> Preview image captures.• Acquire - Capture an image <strong>and</strong> have it automatically transfer to (open in) the third party imagingapplication.312 User Guide to the <strong>SPOT</strong> <strong>Insight</strong> Camera

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