SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

Ch. 15 – Using Other Windows Imaging Applications in Basic ModeTo add, edit, or delete Profiles, right-click on the Profile box. Right clicking gives the following options:• Add – Add a new Profile. When you add a new Profile, the program prompts you to name it.• Save – Save a Profile that you have modified.• Rename – Rename an existing Profile.• Delete - Delete a Profile. This option appears only after you have defined a second Profile in additionto the Default Profile.Exposure: Auto or ManualThe Exposure option gives you the choice of having the camera calculate exposure time (Auto), or settingthe exposure on your own (Manual). The exposure options will vary according to whether you selectAuto or Manual, as illustrated below.ManualAutoWhen you enter one of the above exposure settings, note that it applies both to the live image that you areviewing and the captured image.Auto Exposure optionsImage TypeAn Image Type tells the SPOT program how to expose and process image captures. Each of the 11available Image Types (see below) corresponds to a microscopy technique: select the Image Type thatmatches the technique you are using. The camera will capture the image accordingly. The table belowdescribes typical situations for each image type.Image TypeDescriptionBrightfield-reflected lightPolished metallurgical specimens and semi-conductors using a microscope with avertical illuminator.Brightfield-transmitted lightDarkfield-reflected lightDarkfield-transmitted lightFluorescenceNomarski DIC/HoffmanPhase contrastPolarized lightStained specimens on glass slides using transmitted light microscopes.Bright areas of specimens being viewed with darkfield, reflected light techniques.Bright specimens on dark backgrounds using transmitted light darkfield technique.Fluorescence specimens not fluorescing in a single red, green, or blue primary colorSpecimens being viewed with either Nomarski DIC or Hoffman techniques.Specimens being viewed using the phase contrast technique.Specimens being viewed with polarized light techniques.User Guide to the SPOT Insight Camera 281