SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

Ch. 16 – Using Other Mac Imaging Applicationsseconds. Similarly, the Live Image Maximum option can also be used in cases where the microscope’slight path selector is incorrectly set.Post ProcessingSpecify whether to apply the Post Processing options for the current Image Setup to images captured fromthe live image view.Transferred Images• If the Transferred Images box is selected, the Transfer option applies the Noise Filter, Correct Color,and Image Size Double options as specified for the current Image Setup.• If the Transferred Images box is unselected, the Transfer option ignores the Post Processing options forthe current Image Setup, with one exception: if the Chip Defect Correction option is selected for thecurrent image setup (Enhanced and RT cameras only), the corrections will appear in the image capture,regardless of the Transferred Images preference.Snapped Images• If the Snapped Images box is selected, the Snap option uses all of the Post Processing options from thecurrent Image Setup, except for Background Subtraction and Flatfield Correction.• If the Snapped Images box is unselected, the Snap option ignores the Post Processing options for thecurrent Image Setup, with one exception: if the Chip Defect Correction option is selected for thecurrent image setup (RT and Enhanced cameras only), the corrections will appear in the image capture,regardless of the Snapped Images preference.Palettes (Insight B/W, Insight 3-Shot)The Palettes option allows you to define new palettes and edit existing palettes.SPOT color palettes allow you to apply specific hue and saturation values to monochrome (8 bit or 12 bit)images. Palettes are useful in fluorescence applications where a sample is fluorescing at a singlewavelength, and you want the acquired image to have the correct color. For such a case, you could create apalette to represent the color of the fluorescent dye and then apply that palette to the captured image.There are two ways to apply a palette to an image:• You can associate a palette with an Image Setup, as discussed in Ch. 5 - Preparing to Take a Picture.• You can apply a palette to a captured image by selecting the Set Palette option (Advanced mode) fromthe Edit menu. Refer to Ch. 8 – Viewing, Editing, and Annotating Images for more details.294 User Guide to the SPOT Insight Camera