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SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

SPOT 4.1 Basic and Advanced Software Manual for SPOT Insight ...

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Ch. 16 – Using Other Mac Imaging ApplicationsTo define a new palette, follow these steps:1. From the Setup menu, select Palettes.2. Click on Add. The Color Palette dialogappears as shown here:3. Either use the Hue <strong>and</strong> Saturationselection lists or move the cross-hairs onthe color wheel to enter the values−−Hue is the designation <strong>for</strong> the actualcolor, as distinguished from others inthe color spectrum. Hue is derivedfrom the color wheel <strong>and</strong> is expressedin the color's angular location (indegrees) on the wheel. (e.g., 0° to 360°).Saturation is the proportion of perceived pure hue in the color. Saturation (in the <strong>SPOT</strong> software)is measured on a numerical scale of 0 to 100, where zero is pure gray <strong>and</strong> 100 is pure hue (e.g.,day glow).4. Click on OK.Defining an Image SetupAn image setup serves two crucial purposes:• It defines the way a picture will be taken.• It defines the way the program automatically modifies a picture after it is taken.Image setups are defined to optimize the picture taking process <strong>for</strong> different types of images or microscopytechniques. They can be created, saved, <strong>and</strong> recalled as needed. To define a new image setup, you mustalso select one of 11 pre-defined image types as a template <strong>for</strong> defining the new setup. The 11 pre-definedimage types (in addition to the Factory Defaults setting) correspond to commonly used microscopytechniques. Refer to Ch. 5- Preparing to Take a Picture <strong>for</strong> a table of the different image types. Bracketsaround the title indicate pre-defined image types, while the user defined image setups appear withoutbrackets.• To modify the current image setup, click on the Modify button.• To delete an image setup, select the image setup from the list, <strong>and</strong> click on the Delete button.• To define a new image setup, clickon the Add button. The New ImageSetup window appears with a list ofpre-defined <strong>and</strong> user-definedtemplates (image types). Bracketsindicate pre-defined image types . Select a template, <strong>and</strong> click onOK. The New Image Setup windowappears, as shown at right:User Guide to the <strong>SPOT</strong> <strong>Insight</strong> Camera 295

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