Protein Protocols Protein Protocols

Index 1141
Electrophoresis
of antibodies, 1005–1007
acetic acid urea gels, 103–111
acid-urea triton gels, 113–122
of carbohydrates, 851–858, 898–899
CAT gel electrophoresis, 87–100
detergents for, 134–135
diagonal, 589–593
2D PAGE, 131–139, 141–157, 159–162,
163–168, 169–180, 181–183,
185–196
difference gel electrophoresis, 185–196
gradient SDS PAGE, 69–72, 166
isoelectric focusing, 125–129
low pH, 623–626
non-denaturing PAGE, 57–60
non-equilibrium pH gel electrophoresis,
181–183
peptides, 73–79, 548–550, 553–557,
599–601, 676–679
SDS PAGE, 61–67, 69–72, 73–79
sodium decyl sulfate PAGE, 81–86
use of urea, 134–135
ELISA, 1083–1088
Ellman’s reagent, 483–484, 595–596
Endoglycosidase H, 827
Endoproteinase Glu-C, 513–516, 524–528
Endoproteinase Lys-C, 524–528, 611
Enhanced chemiluminescence (see ECL)
Enzymatic digestion of proteins, 511–521,
523–532, 563–565
Enzyme linked immunosorbent assay (see
ELISA)
Eosin Y stain, 295–297
Ethoxyformylation, 473–474
Eukaryotic cells
lysis of, 1102–1104
radiolabeling of, 159–162
Excoglycosidases, 835, 868, 875–878
F
Fab, 1070
F(ab1 ) 2, 1069–1070
Farmer’s reducer, 267
Ferrozine, ferrous stain, 277, 281
Flicker, 197–218
Flow cytometry for protein quantitation,
45–49
Fluorescein isothiocyanate, 352–354
Fluorochrome labeling, 351–354
Fluorography, 307–312, 627
FPLC, 573
Free radical catalysis, 61
G
β-Galactosidase labeling, 345–346
Gas chromatography, analysis of
monosaccharides, 809–810, 811–812
GC (see Gas chromatography)
Gel electrophoresis (see Electrophoresis)
Gel filtration (permeation) chromatography,
573–578, 995–997
Glutaraldehyde coupling, 958
Glycoproteins
analysis by mass spectrometry, 908–910
characterization by lectin-binding,
795–801, 844–845,917–928
detection in gels and blots, 761–772,
773–776, 779–791
linkages, 780, 784–785, 938
monosaccharide analysis, 768, 805–806,
809–810, 811–812
oligosaccharide release, 817–818,
823–825, 827–828
periodate oxiation of, 803–804
phenol sulfuric acid assay, 803–804
staining, 295–297, 773–776
Gradient SDS PAGE, 69–72, 166
H
Haptens, 953
HAT medium, 1111
Heparan sulfatesaccharides, 893–902
High pH anion exchange chromatography
(see HPAEC)
Histidine, ethoxyformylation of, 473–474
Histones
blotting of, 337–340
electrophoresis of, 103–111, 113–122
Horseradish peroxidase–avidin/streptavidin
conjugates, 417
Horseradish peroxidase–labeled antibodies,
409, 445
Horseradish peroxidase labeling, 347–348
HPAEC analysis of monosaccharides,
768–769, 805–807, 815–816,
831–832, 833–838
HPLC