Protein Protocols Protein Protocols

Index 1143
of glycoproteins, 905–913
mutation detection, 681–692
peptide sequencing, 693–708
protein identification from peptide
masses, 711–730
to detect disulfide linkages, 585–587
use in ladder sequencing, 737–738
MDPF staining of blots, 375–378
Membranes (blotting), 327–328
Membrane staining (see Protein stains)
MEP, hypercell, 1126
Metal chelate stains, 273–285, 287–290
Methionine, cleavage at 485-490
Microwave enhancement of protein assays,
23–30
Molecular weight determination
HPLC, 573–579
mass spectrometry, 683–684
SDS PAGE, 67
Molecular weight standards, 575
Monoclonal antibodies
hybridoma production,1111–1113
hybridoma screening, 1115–1116,
1117–1118
immunization protocols, 1109–1110
murine, 1119–1120, 1129–1136
Monosaccharides
analysis of by HPAEC, 768, 805–806,
815–816, 831–832
analysis by GC, 809–810, 811–812
Murine monoclonal antibodies, 1119–1120,
1129–1136
Mutation detection by mass spectrometry,
681–692
Myeloma cells, 1112, 1133
N
Nanoelectrospray tandem MS, 693–708
NEPHGE, 181–183
Neuraminidase, 801
Nile red stain, 243–248
Nitration of tyrosine, 469–471
Nitric acid method, 31–39
Nitro-thiocyanobenzoate, 503
Non-denaturing PAGE, 57–60
Non-equilibrium pH gel electrophoresis,
181–183
Nucleic acid binding proteins, 81–86
O
O-glycosidase, 827
Oligosaccharides
labeling with ANTS, 875–876
profiling, 819–820, 821–822, 829–830,
831–832
release from glycoproteins, 817–818,
823–825, 827–828
P
PAGE (see Electrophoresis)
Palmitoylation (see Protein palmitoylation)
PAM (see Protein A mimetic)
Papain, 1048
Paper electrophoresis of peptides, 590
Pepsin, 514, 1048
Peptide bond cleavage at
Asn-Gly, 507–510
Asp-X, 499–501
Cys, 503–504
Met, 485-487
Trp, 493–498
Peptide mapping, 533-539, 543–551
Peptide-N-glycosidase F, 827, 854, 868–869
Peptides
antibodies against, 953–961
electrophoresis of, 73–79, 548–550,
553–557, 590, 599–601, 676–679
mapping, 543–551, 553–557, 559–561,
673–679, 686
mass analysis, 711–730, 737–738
phosphopeptides, 673–679
production
by chemical cleavage methods,
485–487, 493–498, 499–501,
503–504, 507–510
by enzymatic methods, 511–521,
523–532
from blots, 523–532
separation of, by HPLC, 533–536,
559–561
sequencing, 693–708
TLC of, 548-550, 676–679
Performic acid oxidation, 457–458, 591
Periodate oxidation of carbohydrates,
803–804
Periodic acid-Schiff stain, 761–765, 775
Phage display, 1059–1069, 1073–1080