Protein Protocols Protein Protocols

Casting IPGs 179
mL of the latter may be measured in a small plastic beaker instead of in a graduated cylinder.
Failure to include glycerol as a humidity-conditioning agent results in slab curling and easy
peeling and tearing of the gel from its support. On washing, IPGs reswell to a different
extent, depending on the pH of the matrix and on the course of the glycerol gradient.
Acknowledgment
The author is most thankful to Ivano Eberini for testing the content of this chapter as
a teaching guide to the use of IPGs.
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