10.12.2012 Views

Protein Protocols Protein Protocols

Protein Protocols Protein Protocols

Protein Protocols Protein Protocols

SHOW MORE
SHOW LESS

Create successful ePaper yourself

Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.

228 Magi et al.<br />

55. Moeremans, M., Daneels, G., and De Mey, J. (1985) Sensitive colloidal metal (gold or silver)<br />

staining of protein blots on nitrocellulose membranes. Analyt. Biochem. 145, 315–321.<br />

56. Daneels, G., Moeremans, M., De Raeymaeker, M., and De Mey, J. (1986) Sequential<br />

immunostaining (gold/silver) and complete protein staining (Aurodye) on Western blots.<br />

J. Immunol. Meth. 89, 89–91.<br />

57. Glenney, J. (1986) Antibody probing of Western blots which have been stained with India<br />

ink. Analyt. Biochem. 156, 315–319<br />

58. Batteiger, B., Newhall, W. J. V., and Jones, R. B. (1982) The use of Tween 20 as a blocking<br />

agent in the immunological detection of proteins transferred to nitrocellulose membrane.<br />

J. Immunol. Meth. 55, 297–307.<br />

59. Fultz, C. D. and Witzmann, F. A. (1997) Locating Western blotted and immunostained<br />

proteins within complex two-dimensional patterns. Analyt. Biochem. 251, 288–291.<br />

60. Zeindl-Eberhart, E., Jungblut, P. R, and Rabes, H. M. (1997) A new method to assign<br />

immunodetected spots in the complex two-dimensional electrophoresis pattern. Electrophoresis<br />

18, 799–801.<br />

61. Johansson, K. E. (1986) Double replica electroblotting: a method to produce two replicas<br />

from one gel. J. Biochem. Biophys. Meth. 13, 197–203.<br />

62. Neumann, H. and Mullner, S. (1998) Two replica blotting methods for fast immunological<br />

analysis of common proteins in two-dimensional electrophoresis. Electrophoresis<br />

19, 752–757.<br />

63. Gotzmann, J. and Gerner, C. (2000) A method to produce Ponceau replicas from blots:<br />

application for Western analysis. Electrophoresis 21, 523–525.<br />

64. Kamps, M. P. (1991) Generation of anti-phosphotyrosine antibodies for immunoblotting.<br />

Meth. Enzymol. 201, 101–110.<br />

65. Michalewski, M. P., Kaczmarski, W., Golabek, A., Kida, E., Kaczmarski, A., and<br />

Wisniewski, K. E. (1999) Immunoblotting with antiphosphoaminoacid antibodies: importance<br />

of the blocking solution. Analyt. Biochem. 276, 254–257.<br />

66. Towbin, H. and Gordon, J. (1984) Immunoblotting and dot immunobinding. Current status<br />

and outlook. J. Immunol. Meth. 72, 313–340.<br />

67. Stott, D. I. (1989) Immunoblotting and dot blotting. J. Immunol. Meth. 119, 153–187.<br />

68. Poxton, I. R. (1990) Immunoblotting techniques. Curr. Opin. Immunol. 2, 905–909.<br />

69. Harper, D. R., Ming-Liu, K., and Kangro, H. O. (1990) <strong>Protein</strong> blotting: ten years on.<br />

J. Virol. Meth. 30, 25–40.<br />

70. Mohammad, K. and Esen, A. (1989) A blocking agent and a blocking step are not needed in<br />

ELISA, immunostaining dot-blots and Western blots. J. Immunol. Meth. 117, 141–145.<br />

71. Flanagan, S. D. and Yost, B. (1984) Calmodulin-binding proteins: visualization by 125 I-calmodulin<br />

overlay on blots quenched with Tween 20 or bovine serum albumin and<br />

poly(ethylene oxide). Analyt. Biochem. 140, 510–519.<br />

72. Hoffman, W. L. and Jump, A. A. (1986) Tween 20 removes antibodies and other proteins<br />

from nitrocellulose. J. Immunol. Meth. 94, 191–197.<br />

73. Sadra, A., Cinek, T., and Imboden, J. B. (2000) Multiple probing of an immunoblot membrane<br />

using a non-block technique: advantages in speed and sensitivity. Analyt. Biochem.<br />

278, 235–237.<br />

74. Krajewski, S., Zapata, J. M., and Reed, J. C. (1996) Detection of multiple antigens on<br />

Western blots. Analyt. Biochem. 236, 221–228.<br />

75. Bhatt, T. R., Taylor III, P. A., and Horodyski, F. M. (1997) Suppression of irrelevant signals<br />

in immunoblots by preconjugation of primary antibodies. BioTechniques 23, 1006–1010.<br />

76. Wilkinson, D. (2000) Chemiluminescent techniques for Western blot detection let<br />

researcher shed their lead aprons. The Scientist 14, 29–32.

Hooray! Your file is uploaded and ready to be published.

Saved successfully!

Ooh no, something went wrong!