Protein Protocols Protein Protocols

846 Wong et al.
Table 1
Theoretical Tryptic Peptides of Thy-1 in Order of Increasing
Hydrophobic Character
HPLC a Residues Sequences b
–15.4 179–99 K T Fx 2–3
–12.9 189–99 R T complex
–7.9 138–49 R K chain on
–4.7 189–105 R D N 98 KT
–4.2 138–39 R K
–2.6 111–111 K
–1.1 157–58 R V
–0.8 140–41 K H
–0.0 106–107 R L
–2.7 111–2 V
2.9 140–40 K K
2.9 141–41 K H
4.1 179–85 K V
8.2 100–107 K L
10.9 117–20 R H
14.8 100–105 K D
17.2 108–111 K Fx 8–9
19.8 106–110 R C Possible GPI
24.8 121–39 R K Anchor at
25.3 157–65 R F VKC- or high
26.4 108–110 K C Man at N23 NT
28.7 166–68 R V
33.0 159–65 R F
35.6 121–37 R E Fx 10
37.1 142–58 K V High Man
39.9 117–37 R E at N 23 NT
41.1 141–56 K S
44.8 142–56 K S
55.1 159–68 R V
55.8 111–16 L
59.7 113–16 R L
64.0 113–26 R H
67.1 169–88 K V Fx 13–14
69.6 169–78 K D Complex and
hybrid at N 74 FT
91.7 166–78 R D
a Relative hydrophobicity index on C18 HPLC.
b < > denotes the possible alternative tryptic cleavage sites.