Protein Protocols Protein Protocols

1144 Index
Phenol-sulfuric acid assay, 803–804
Phosphopeptides, 673–679
Phosphopeptide mapping, 673–679
Phosphor imaging, 222
Phosphorylation (see Protein
phosphorylation)
Photopolymerization, 116, 126–128
PMSF, 1099
Polyacrylamide gels (see also Protein stains
and Protein blotting)
electroelution, 299–304
formation of, 61
fractionation range of, 59, 66–67
staining of proteins in, 238–239,
243–248, 251–257, 259–262,
233–234, 265–270, 295–297,
280–290
structure of, 62
Polyacrylamide gel electrophoresis (see
Electrophoresis)
Polyclonal antibodies (see Antibodies and
IgG)
Polyethylene glycol, purification of IgG,
991
Polyvinyl alcohol, 412
Ponceau S stain, 218, 390
Porous graphitized carbon (PGC), 819–820,
829–830
Prolidase, 564
Pronase,563–564
Pronase E, 669
Pro-Q Emerald 300 dye staining, 767–768
Protease inhibitors, 1099
Protein A, 406, 993
Protein A mimetic, 1013–1023
Protein G, 406, 410, 993
Protein L, 406, 410, 993
Protein LA, 405, 410, 993
Proteins
protein blotting, 76-77, 215–224,
317–319, 321–333, 335–336,
337–340
acid-urea gels, 337–340
blocking solutions, 218, 223, 330–332
blotting (transfer) buffer, 217, 221,
324–326
capillary, 335–336
detection by chemifluorescence,
421-428
detection by ECL, 218–219, 429–436
detection with antibodies, 405–413
detection with avidin-biotin, 415–418
digestion of proteins on, 523–532, 676
double relica blotting, 222
electroblotting, 317–319, 337–340
membranes, 327–328
re-use of blots, 439–451
semidry, 321–333
staining of blots, 218, 273–285, 276–279,
375–378, 387–391, 393–403
cross-linking, 367–369
DNA cross linking, 747, 753–757
digestion, 511–516, 563–565
kinases, 605
ladder sequencing, 587, 733–738
molecular weight standards, 575
palmitoylation, 633–638
phosphorylation, 603–608, 609–621,
673–679
prenylation, 641–654, 657–669
quantitation of
BCA assay, 11–14, 23–30
Bradford method, 15–20
copper iodide, 381–385
flow cytometry, 45–49
in gels, 231–235, 237–242
kinetic silver staining, 51–54
Lowry method, 7–9, 23–30
nitric acid method, 31–39
on blots, 429–436
radiolabeled, 231–235
UV absorbance, 3–6
radiolabeling, 369–370, 546–547,
963–964, 967–968, 969–970,
971–975
stains (gels and blots)
Alcian blue, 773–776
amido black, 390
aurodye forte, 398–399
auroprobe BL plus, 397–398
calconcarboxylic acid, 259–262
Coomassie brilliant blue, 58, 64, 67,
94, 390
Coomassie brilliant blue G, 238–240
copper iodide, 381–385
DIG-Anti-DIG AP labeling, 761–765
direct blue,71
Eosin Y, 295–297