Protein Protocols Protein Protocols

Enzymatic Digestion 521
11. Modification of cysteine residues with MNS provides the advantage that the resulting
S-methyl cysteine phenylthiohydantoin derivative elutes in a favorable position (i.e.,
between PTH-Tyr and PTH-Pro) using an Applied Biosystems/Perkin Elmer PTH C-18
column. Either the Applied Biosystems Premix or sodium acetate buffer system can be
used, with a linear gradient extending from 10 to 38% buffer B over 27 min.
References
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