Protein Protocols Protein Protocols

498 Smith
References
1. Kilic, F. and Ball, E. H. (1991). Partial cleavage mapping of the cytoskeletal protein
vinculin. Antibody and talin binding sites. J. Biol. Chem. 266, 8734–8740.
2. Litchfield, D. W., Lozeman, F. J., Cicirelli, M. F., Harrylock, M., Ericsson, L. H., Piening,
C. J., and Krebs. E. G. (1991) Phosphorylation of the beta subunit of casein kinase II in
human A431 cells. Identification of the autophosphorylation site and a site phosphorylated
by p34cdc2. J. Biol. Chem. 266, 20,380–20,389.
3. Tseng, A., Buchta, R., Goodman, A. E., Loughman, M., Cairns D., Seilhammer, J., et al.
(1991) A strategy for obtaining active mammalian enzyme from a fusion protein expressed
in bacteria using phospholipase A2 as a model. Pro. Express. and Purificat. 2, 127–135.
4. Huang, H. V., Bond, M. W., Hunkapillar, M. W., and Hood, L. E. (1983) Cleavage at
tryptophanyl residues with dimethyl sulfoxide-hydrochloric acid and cyanogen bromide.
Meth. Enzymol. 91, 318–324.
5. Rosa, J. C., de Oliveira, P. S. L., Garrat, R., Beltramini, L., Roque-Barreira, M.-C. and
Greene, L. J. (1999) KM+, a mannose-binding lectin from Artocarpus integrifolia: amino
acid sequence, predicted tertiary structure, carbohydrate recognition, and analysis of the
beta-prism fold. Prot. Sci. 8, 13–24.
6. Beavis, R. C. and Chait. B. T. (1990). Rapid, sensitive analysis of protein mixtures by
mass spectrometry. Proc. Natl. Acad. Sci. USA 87, 6873–6877.
7. Morrison, J. R., Fidge, N. H., and Grego, B. (1990) Studies on the formation, separation,
and characterisation of cyanogen bromide fragments of human A1 apolipoprotein. Analyt.
Biochem. 186, 145–152.
8. Ozols, J. and Gerard, C. (1977). Covalent structure of the membranous segment of horse
cytochrome b5. Chemical cleavage of the native hemprotein. J. Biol. Chem. 252, 8549–8553.
9. Savige, W. E. and Fontana, A. (1977) Cleavage of the tryptophanyl peptide bond by dimethyl
sulfoxide-hydrobromic acid. Meth. Enzymol. 47, 459–469.
10. Fontana, A. (1972) Modification of tryptophan with BNPS skatole (2-(2-nitro-phenylsulfenyl)-
3-methyl-3'-bromoindolenine). Meth. Enzymol. 25, 419–423.
11. Crimmins, D. L., McCourt, D. W., Thoma, R. S., Scott, M. G., Macke, K. and Schwartz,
B. D. (1990) In situ cleavage of proteins immobilised to glass-fibre and polyvinylidene
difluoride membranes: cleavage at tryptophan residues with 2-(2'-nitropheylsulfenyl)-
3-methyl-3'-bromoindolenine to obtain internal amino acid sequence. Analyt. Biochem.
187, 27–38.
12. Ramachandran, L. K. and Witkop, B. (1976). -Bromosuccinimide cleavage of peptides.
Meth. Enzymol. 11, 283–299.
13. Lischwe, M. A. and Sung, M. T. (1977). Use of -chlorosuccinimide/urea for the selective
cleavage of tryptophanyl peptide bonds in proteins. J. Biol. Chem. 252, 4976–4980.
14. Lischwe, M. A. and Ochs, D. (1982). A new method for partial peptide mapping using
chlorosuccinimide/urea and peptide silver staining in sodium dodecyl sulphate-polyacrylamide
gels. Analyt. Biochem. 127, 453–457.
15. Fontana, A., Dalzoppo, D., Grandi, C., and Zambonin, M. (1983) Cleavage at tryptophan
with iodosobenzoic acid. Meth. Enzymol. 91, 311–318.
16. Vestling, M. M., Kelly, M. A., and Fenselau, C. (1994) Optimization by mass spectrometry
of a tryptophan-specific protein cleavage reaction. Rapid Commun. Mass Spectr. 8,
786–790.
17. Rahali, V. and Gueguen, J. (1999) Chemical cleavage of bovine (β-lactoglobulin by BNPSskatole
for preparative purposes: comparative study of hydrolytic procedures and peptide
characterization. J. Prot. Chem. 18, 1–12.