Protein Protocols Protein Protocols

Immunogold 403
15. AuroDye forte is designed for use on nitrocellulose and PVDF membranes. It cannot be
used on nylon membranes where its charge will result in staining of the whole membrane
(see Table 3).
References
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Immunochemistry 8, 1081.
2. Romano, E. L., Stolinski, C., and Hughes-Jones, N. C. (1974) An immunoglobulin reagent
labelled with colloidal gold for use in electron microscopy. Immunocytochemistry 14,
711–715.
3. Horisberger, M. and Rosset, J. (1977) Colloidal gold, a useful marker for transmission and
scanning electron microscopy. J. Histochem. Cytochem. 25, 295–305.
4. Roth, J. (1982) Applications of immunocolloids in light microscopy: preparation of protein
A-silver and protein A-gold complexes and their applications for the localization of
single and multiple antigens in paraffin sections. J. Histochem. Cytochem. 30, 691–696.
5. Moeremans, M., Daneels, G., Van Dijck, A., Langanger, G., and De Mey, J. (1984) Sensitive
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bound to antigens on nitrocellulose by protein A-gold complexes. Analyt. Biochem.
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Biochem. 142, 221–225.
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the sulphide silver method suitable for both light and electron microscopy. Histochemistry
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9. Geoghegan, W. D. and Ackerman, G. A. (1977) Adsorption of horseradish peroxidase, ovomucoid
and anti-immunoglobulin to colloidal gold for the indirect detection of concanavilin
A, wheat germ agglutinin and goat antihuman immunoglobulin G on cell surfaces at the
electron microscopic level: a new method, theory and application. J. Histochem. Cytochem.
25, 1182–1200.
10. Western Blotting Technical Manual, Amersham International plc. 1991, Amersham UK.
11. Moeremans, M., Daneels, G., De Raeymaeker, M., and Leunissen, J. L. M. (1989)
AuroProbe One in immunoblotting, in Aurofile 02, Janssen Life Sciences, Wantage, UK,
pp. 4, 5.
12. Moeremans, M., Daneels, G., and DeMey, J. (1985) Sensitive colloid (gold or silver) staining
of protein blots on nitrocellulose membrane. Analyt. Biochem. 145, 315–321.
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15. Chevallet, M., Procaccio V., and Rabilloud, R. (1997) A non-radioactive double detection
method for the assignment of spots in two-dimensional blots. Analyt. Biochem. 251, 69–72.
16. Egger, D. and Bienz, K. (1987) Colloidal gold staining and immunoprobing of proteins on
the same nitrocellulose blot. Analyt. Biochem. 166, 413–417.
17. Egger, D. and Bienz, K. (1992) Colloidal gold staining and immunoprobing on the same
Western blot, in Methods in Molecular Biology, Vol. 10, Immunochemical Protocols
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