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Preparation of Avidin Conjugates 373<br />

satisfactory. Radiolabeled streptavidin is aliquoted (~100 µL/tube) and stored at 4°C or<br />

–20°C until use.<br />

References<br />

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Edsall, J. T., and Richards, F. M., eds.), Academic Press, New York, pp. 85–133.<br />

2. Bayer, E. A. and Wilchek, M. (1980) The use of the avidin-biotin complex as a tool in<br />

molecular biology. Meth. Biochem. Analyt. 26, 1–45.<br />

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directed self-assembly of proteins: semisynthetic DNA-streptavidin hybrid molecules as<br />

connectors for the generation of macroscopic arrays and the construction of supramolecular<br />

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(1981) Iodination of proteins, glycoproteins, and peptides using a solid-phase oxidizing agent,<br />

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8. Mock, D. M. (1990) Sequential solid-phase assay for biotin based on 125I-labeled avidin in<br />

Meth. Enzymol. 184, 224–233.<br />

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by conjugation to an 125I-containing acylating agent. Applications to the radioimmunoassay.<br />

Biochem. J. 133, 529–539.<br />

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using N-succinimidyl 4-hydroxy-3-iodobenzoate. Bioconjugate Chem. 4, 78–84.<br />

11. Vaidyanathan, G. and Zalutsky, M. R. (1990) Radioiodination of antibodies via N-succinimidyl<br />

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L. M. (1991) Preparation and in vivo evaluation of an N-(p-[ 125I]iodophenethyl)maleimide antibody conjugate. Bioconjugate Chem. 2, 435–440.<br />

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