Protein Protocols Protein Protocols

656 Corsini
24. Danesi, R., McLellan, C. A., and Myers, C. E. (1995) Specific labeling of isoprenylated
proteins: application to study inhibitors of the posttranslational farnesylation and
geranylgeranylation. Biochem. Biophys. Res. Commun. 206, 637–643.
25. Shin-ichi, O., Watanabe, M., and Nishino,T. (1996) Identification and characterization of
geranylgeraniol kinase and geranylgeranyl phosphate kinase from the Archebacterium
Sulfolobus acidocaldarius. J. Biochem. 119, 541–547.
26. Westfall, D., Aboushadi, N., Shackelford, J. E., and Krisans, S. (1997) Metabolism of
farnesol: phosphorylation of farnesol by rat liver microsomial and peroxisomal fractions.
Biochem. Biophys. Res. Commun. 230, 562–568.
27. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951) Protein measurement
with the folin phenol reagent. J. Biol. Chem. 193, 265–275.
28. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of
bacteriophage T4. Nature 227, 680–685.
29. Görg, A., Postel, W., and Günther, S. (1988) The current state of two-dimensional electrophoresis
with immobilized pH gradients. Electrophoresis 9, 531–546.
30. Jungblutt, P., Thiede, B., Simny-Arndt, U., Müller, E.-C., Wittmann-Liebold, B., and
Otto, A. (1997) Resolution power of two-dimensional electrophoresis and identification
of proteins from gels. Electrophoresis 17, 839–847.
31. O’Farrell, P. (1975) High resolution two-dimensional electrophoresis of proteins. J. Biol.
Chem. 250, 4007–4021.
32. Patton, W. F., Pluskal, M. G., Skea, W. M., Buecker, J. L., Lopez, M. F., Zimmermann, R.,
Lelanger, L. M., and Hatch, P. D. (1990) Development of a dedicated two-dimensional gel
electrophoresis system that provides optimal pattern reproducibility and polypeptide
resolution. BioTech. 8, 518–529.
33. Lopez, M. F. and Patton, W. F. (1990) Reproducibility of polypeptide spot positions in
two-dimensional gels run using carrier ampholytes in the isolelectric focusing dimension.
Electrophoresis 18, 338–343.
34. Görg, A., Günther, B., Obermaier, C., Posch, A., and Weiss, W. (1995) Two-dimensional
polyacrylamide gel electrophoresis with immobilized pH gradients in the first dimension
(IPG-Dalt): the state of the art and the controversy of vertical versus horizontal systems.
Electrophoresis 16, 1079–1086.
35. Schmidt, R. A., Schneider, C. J., and Glomset, J. A. (1984) Evidence for post-translational
incorporation of a product of mavalonic acid into Swiss 3T3 cell proteins. J. Biol. Chem.
259, 10,175–10,180.
36. Sanchez, J.-C., Rouge, V., Pisteur, M., Ravier, F., Tonella, L., Moosmayer, M., Wilkins, M. R.,
and Hochstrasser, D. F. (1997) Improved and simplified in-gel sample application using
reswelling of dry immobilized pH gradients. Electrophoresis 18, 324–327.
37. Huber, L. A., Ullrich, O., Takai, Y., Lütcke, A., Dupree, P., Olkkonen, et al. (1994) Mapping
of Ras-related GTP-binding proteins by GTP overlay following two-dimensional gel
electrophoresis. Proc. Natl. Acad. Sci. USA 91, 7874–7878.