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Advances in Fingerprint Technology.pdf

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would be of <strong>in</strong>terest, if future fund<strong>in</strong>g is available, to evaluate the impact of<br />

other environmental conditions on latent pr<strong>in</strong>t decomposition products and<br />

rate.<br />

DNA From Latent Pr<strong>in</strong>ts<br />

Another important component of latent pr<strong>in</strong>t residue is deoxyribonucleic<br />

acid (DNA). It is not surpris<strong>in</strong>g that a significant amount of DNA is often<br />

present <strong>in</strong> visible blood pr<strong>in</strong>ts. However, it can also be deposited <strong>in</strong> nonblood<br />

latent pr<strong>in</strong>t residue from the epidermal cells that are cont<strong>in</strong>uously<br />

sloughed off the sk<strong>in</strong> surface through rubb<strong>in</strong>g of the sk<strong>in</strong> or through direct<br />

contact with a substrate. In the past, an exam<strong>in</strong>er was often forced to decide<br />

what evidence is more important, the DNA or the ridge detail. <strong>Advances</strong> <strong>in</strong><br />

DNA technology have made this decision easier s<strong>in</strong>ce fewer latent pr<strong>in</strong>t<br />

visualization processes <strong>in</strong>hibit sample analyses. The use of polymerase cha<strong>in</strong><br />

reaction (PCR) analysis has allowed subnanogram quantities of DNA to be<br />

detected, amplified, and analyzed. In addition, “lab on a chip” technology<br />

will soon allow for extremely fast analysis and identification at the crime<br />

scene. 122-125 Exam<strong>in</strong>ers are now also able to extract DNA <strong>in</strong> situations previously<br />

considered improbable. Sweet et al. reported that identifiable DNA was<br />

obta<strong>in</strong>ed from a bite mark on sk<strong>in</strong> from a victim who had been drowned. 126<br />

Such advances will beg<strong>in</strong> to highlight the need to rapidly and reliably extract,<br />

analyze, and identify DNA recovered from latent pr<strong>in</strong>ts.<br />

DNA From Blood Pr<strong>in</strong>ts and Sta<strong>in</strong>s<br />

The recovery of DNA from visible blood pr<strong>in</strong>ts and latent blood pr<strong>in</strong>ts<br />

developed by chemical reagents has been well documented. Most studies<br />

found that only a few visualization reagents <strong>in</strong>hibit DNA analysis. A study<br />

of envelopes, stamps, and cigarette butts by Presley et al. us<strong>in</strong>g Chelex extraction<br />

and PCR HLA DQ alpha typ<strong>in</strong>g found negative DNA results after process<strong>in</strong>g<br />

with PD. 127 A subsequent study by Walls also found that physical<br />

developer adversely affected DNA analysis. 128 However, it was reported that<br />

the problem with PD could be overcome by us<strong>in</strong>g organic extraction rather<br />

than Chelex. Ste<strong>in</strong> et al. studied the effect of black powder, n<strong>in</strong>hydr<strong>in</strong>,<br />

cyanoacrylate fum<strong>in</strong>g, and gentian violet on 1-, 14-, and 56-day-old bloodsta<strong>in</strong>s<br />

and saliva samples. They found that none of the latent pr<strong>in</strong>t visualization<br />

treatments adversely affected DNA extraction, quality, or typ<strong>in</strong>g us<strong>in</strong>g<br />

restriction fragment length polymorphism (RFLP) or PCR-short tandem<br />

repeat (STR). 129 Another study exam<strong>in</strong>ed the effects of cyanoacrylate (CA)<br />

fum<strong>in</strong>g and forensic light sources on bloodsta<strong>in</strong>s with subsequent analysis

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