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Advances in Fingerprint Technology.pdf

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Procedure<br />

Xylene 50 mL<br />

Petroleum ether 830 mL<br />

1. Dip the paper conta<strong>in</strong><strong>in</strong>g latent pr<strong>in</strong>ts <strong>in</strong>to the freshly prepared solution<br />

for 5 sec.<br />

2. Allow the paper specimen to dry for 30 sec.<br />

3. Repeat the dipp<strong>in</strong>g for another 5 sec (some <strong>in</strong>dicate this step can be<br />

omitted)<br />

4. Heat the paper to 100°C for 10 m<strong>in</strong>.<br />

5. View the surface under alternative light sources as follows:<br />

a. Video spectral comparator (VSC-1) us<strong>in</strong>g blue-green light<br />

excitation — Fluorescence can be observed through a 610-nm filter.<br />

b. A 12-W argon laser operated at 514 nm — Fluorescence can be<br />

observed through a 550- or a 610-nm filter.<br />

c. A mercury vapor lamp — Fluorescence can be observed through a<br />

546-nm filter.<br />

As noted above, Jungbluth 118 formulated DFO <strong>in</strong> a Freon substitute: 50 mg<br />

DFO is dissolved <strong>in</strong> 4 mL methanol and 2 mL acetic acid; then 94 mL Genesolv<br />

2000 is added. Geide 143 similarly formulated DFO <strong>in</strong> the Freon-substitute<br />

solvent t-butyl-methyl ether. He noted that the solvent is highly flammable<br />

and volatile and must be used with care <strong>in</strong> a hood. Bratton and Juhala 144<br />

described a sandwich<strong>in</strong>g procedure <strong>in</strong> which the test item was sandwiched<br />

between DFO-treated filter papers and then subjected to 5% acetic acid<br />

“steam” (from a steam iron), to get around the problem of solvent-caused<br />

<strong>in</strong>k bleed on documents.<br />

N<strong>in</strong>hydr<strong>in</strong> Analogues<br />

Almog, Hirschfeld, and Klug 145 synthesized several n<strong>in</strong>hydr<strong>in</strong> analogues:<br />

benzo[e]n<strong>in</strong>hydr<strong>in</strong> (2,2-dihydroxybenz[e]-<strong>in</strong>dane-1,3-dione), benzo[f]n<strong>in</strong>hydr<strong>in</strong><br />

(2,2-dihydroxybenz[f]-<strong>in</strong>dane-1,3-dione), and 2,2-dihydroxy-<br />

5-chloro-6-methoxy<strong>in</strong>dane-1,3-dione. These compounds were tested for<br />

their applicability to latent pr<strong>in</strong>t detection. It was found that the n<strong>in</strong>hydr<strong>in</strong><br />

analogues developed latent pr<strong>in</strong>ts with a sensitivity similar to that of n<strong>in</strong>hydr<strong>in</strong><br />

and that the quality of development was <strong>in</strong>dependent of the age of the<br />

latent f<strong>in</strong>gerpr<strong>in</strong>ts. Benzo[f]n<strong>in</strong>hydr<strong>in</strong> performance <strong>in</strong> several solvent formulations<br />

was recently compared directly with n<strong>in</strong>hydr<strong>in</strong>, and found to be<br />

superior for certa<strong>in</strong> surfaces, but not <strong>in</strong> the number of pr<strong>in</strong>ts developed on<br />

actual exhibits overall. 146

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