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METHODS<br />

In the spring and summer of 1993, we tested the response of five species of bark beetles to methyl chavicol in<br />

Calif<strong>or</strong>nia and Idaho. In April and May, we tested western pine beetle, red turpentine beetle, and two species of Ips, L<br />

paraconfusus and I. pini, at the University of Calif<strong>or</strong>nia's Blodgett F<strong>or</strong>est <strong>Research</strong> <strong>Station</strong> in the central Sierra Nevada.<br />

Tests were conducted as described previously (Hobson 1992, Hobson et al. 1993). F<strong>or</strong> each beetle species, attractive lures<br />

were placed on lindgren traps. In each test there were four treatments" (1) attractant, (2) attractant and methyl chavicol, (3)<br />

methyl chavicol alone, and (4) control blank.<br />

Beetles were collected daily and treatments were randomized after each collection. All four treatments were replicated<br />

in 4-10 blocks. Methyl chavicol was released from four open 1.5-ml epend<strong>or</strong>f tubes at a rate of 0.5 ml/day. The<br />

attractant f<strong>or</strong> D. valens was a set with four 1.5-ml epend<strong>or</strong>f tubes of S(-)[3-pinene. F<strong>or</strong> the western pine beetle, I. pini and I.<br />

paraconfusus commercial lures provided by Phero Tech (Delta, BC) were used. Western pine beetle lures contained exobrevicomin,<br />

frontalin and myrcene. I. pini lures contained ipsdienol (3%+/97%-). I. paraconfusus lures were ipsenol (50%+/<br />

50%-), ipsdienol (97%+/3%-), and cis-verbenol.<br />

In July and August of 1993, we tested mountain pine beetle and I. pini in the Sawtooth National Recreation Area in<br />

south central Idaho. The experimental design was as above. Attractant lures f<strong>or</strong> mountain pine beetle (trans-verbenol, exobrevicomin<br />

and myrcene) were obtained from Phero Tech. Attractant lures f<strong>or</strong> I. pini were as above.<br />

RESULTS<br />

Methyl chavicol significantly reduced attraction of western pine beetle and mountain pine beetle to their respective<br />

aggregation pheromones (Table 1). Attraction of D. valens to [3-pinene was not significantly interrupted by methyl chavicol.<br />

L pini catch was reduced by 29%, but <strong>this</strong> was not significant. I. paraconfusus flew too late in Calif<strong>or</strong>nia f<strong>or</strong> us to collect<br />

adequate data f<strong>or</strong> statistical analysis; however, in five of six blocks where I. paraconfusus was collected, traps baited with<br />

methyl chavicol and the bait had fewer beetles than traps with bait alone.<br />

Table 1.--Bark beetle response to Methyl chavicol<br />

Pheromone Pheromone & Percent<br />

baifi SD methyl chavicol _ SD reduction<br />

Western pine beetle 53.5 a2 69.7 21.6b 28.0 60<br />

Mountain pine beetle 27.4c 47.5 7.9d 12.4 71<br />

Red turpenti ne beetle 10.6 6.28 9.06 6.8 14<br />

Ips pini 20.5 19.7 14.5 12.2 29<br />

_Meancatch.<br />

2Numbersin a row followed by different letters are significantly different o_= 0.05 ANOVA followed by Wilcoxon test.<br />

229

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