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4 CYTOKINE SIGNALING Cytokine Genes 104 Cytokine Antibodies 104 Cytokine Reporter Cells 105-116
CYTOKINE SIGNALING 4 Cytokine and Related Genes Cytokines are key modulators of the immune system. They are produced mainly by T lymphocytes in response to infectious agents. Some cytokines induce the inflammatory response or stimulate B cells to produce antibodies while others act to offset these effects by downregulation. InvivoGen offers a comprehensive list of cytokine genes classified in different families including interleukins and chemokines. Description Cytokine and related genes are provided either in a pORF or a pUNO plasmid (see page 37), both containing the complete coding sequence from the ATG to the Stop codon. Each gene is fully sequenced. Gene families in pORF plasmids: - Chemokine genes - Immune receptor genes - Chemokine receptor genes - Interferon genes - Cytokine genes - Interleukin genes - Cytokine suppressor genes - Interleukin receptor genes Gene family in pUNO plasmids: - Interferon signaling genes Contents and Storage Each pORF and pUNO plasmid is provided as a lyophilized transformed E. coli strain on a paper disk. Transformed strains are shipped at room temperature and should be stored at -20°C. Each pORF is provided with 4 pouches of E. coli Fast-Media ® Amp (2 TB and 2 Agar) and each pUNO plasmid with 4 pouches of E. coli Fast-Media ® Blas (see pages 44-45). PRODUCT QUANTITY CAT. CODE* pORF- E. coli disk porf- pUNO- E. coli disk puno- * Catalog codes are available on our website Anti-Cytokine Neutralizing IgA Antibodies Description Neutralizing IgA antibodies are chimeric monoclonal antibodies in which the constant domains of the human IgA molecule were combined with murine variable regions. They have been selected for their ability to efficiently neutralize the biological activity of selected cytokines. The neutralizing activity of these IgA antibodies was determined using InvivoGen’s HEK-Blue Cytokine Cells. Contents and Storage Each neutralizing IgA antibody is provided lyophilized from a 0.2 μm filtered solution in PBS. Product should be reconstituted in sterile water. Lyophilized antibodies are stable greater than six months when stored at -20ºC. Reconstituted IgAs are stable 1 month when stored at 4ºC and 6 months when aliquoted and stored at -20ºC. PRODUCT ANTIGEN REACTIVITY QUANTITY CATALOG CODE Anti-hCD40L-IgA2 Human CD40 ligand Human 100 μg maba-h40l Anti-hIFNa-IgA2 Human Interferon a Human 100 μg maba-hifna Anti-hIFNg-IgA2 Human Interferon g Human 100 μg maba-hifng Anti-hIL-1b-IgA2 Human Interleukin 1b Human 100 μg maba-hil1b Anti-hIL-4-IgA2 Human Interleukin 4 Human 100 μg maba-hil4 Anti-hIL-6-IgA2 Human Interleukin 6 Human 100 μg maba-hil6 Anti-hIL-13-IgA2 Human Interleukin 13 Human 100 μg maba-hil13 Anti-hIL-18-IgA2 Human Interleukin 18 Human 100 μg maba-hil18 Anti-hTGFb-IgA2 Human Tumor Growth Factor b Human 100 μg maba-htgfb Anti-hTNFa-IgA2 Human Tumor Necrosis Factor a Human 100 μg htnfa-mab7 104 www.invivogen.com/cytokine-signaling
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INNOVATION WITHIN REACH InvivoGen
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CONTENTS AT A GLANCE 1 CELL CULTURE
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4 CYTOKINE SIGNALING TABLE of CONTE
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Mycoplasma Detection & Elimination
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PlasmoTest Contents - HEK-Blue -2
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Primocin - The Antimicrobial Shiel
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SEAP Reporter Gene System Secreted
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HEK-Blue Detection - Real-Time Det
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Blasticidin S Description Blasticid
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LyoVec New formulation LyoVec is
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Poly-Arginine-HA Tagged Reprogrammi
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LENTI-Smart - Lentiviral Vector Pr
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Lentiviral Vector Production and Ce
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Antibody generation using pFUSE-CHI
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Engineering Fc regions for altered
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pBROAD & pWHERE - Optimized Vector
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pSELECT-Tag - Expression of a GFP-,
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pVIVO - Expression of Two Genes of
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pORF Description pORF plasmids feat
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Prom A-List - Human and Rodent Pro
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PROMOTER GENE TISSUE DISTRIBUTION S
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ChemiComp E. coli ChemiComp E. coli
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Fast-Media ® Preparation 1. Empty
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RIG-I-like Receptors 54 RLR & RLR-R
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Toll-Like Receptors Toll-Like Recep
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www.invivogen.com/innate-immunity 5
Page 54 and 55: IPAF and NAIP5 IPAF (NLRC4, CLAN/CA
Page 56 and 57: C-Type Lectin Receptors C-type lect
Page 58 and 59: pUNO pUNO-HA Toll-Like Receptors (T
Page 60 and 61: GENE NAME/ALIASES DESCRIPTION CAT.
Page 62 and 63: pDUO - Gene Associations Features a
Page 64 and 65: pZERO-TLR & pDeNy - Dominant Negati
Page 66 and 67: 293/TLR & 293/NOD Clones 293/TLR Cl
Page 68 and 69: Response of HEK-Blue hTLR2 cells t
Page 70 and 71: Ramos-Blue Cells - NF-kB/AP-1 Repo
Page 72 and 73: MEF Cells C3H/TLR4mut & C3H/WT MEFs
Page 74 and 75: Antibodies for Detection ANTIBODY D
Page 76 and 77: - NF-kB binding site: Nuclear facto
Page 78 and 79: PRODUCT ORIGIN/DESCRIPTION TLR2 Ago
Page 80 and 81: PRODUCT ORIGIN/DESCRIPTION TLR9 Ago
Page 82 and 83: PRODUCT ORIGIN/DESCRIPTION Dectin-1
Page 84 and 85: LPS-PG (Porphyromonas gingivalis) R
Page 86 and 87: • FLA-ST ultrapure was purified b
Page 88 and 89: 1. Zhao H. et al., 2004. Contributi
Page 90 and 91: 1. Hornung V. et al., 2006. 5'-Trip
Page 92 and 93: TLR/NOD Ligand Screening InvivoGen
Page 94 and 95: Signal Transduction Inhibitors Invi
Page 96 and 97: Pepinh-MYD - MyD88 inhibitory pepti
Page 98 and 99: 1. Martinon F,. et al., 2002. The i
Page 100 and 101: Nigericin - NLRP3 Inflammasome Indu
Page 102 and 103: Autophagy is a pathway by which cyt
Page 106 and 107: Blue Cytokine Reporter Cells Blue
Page 108 and 109: HEK-Blue IFN-a/b Cells - Human Typ
Page 110 and 111: HEK-Blue IL-6 Cells - IL6 Reporter
Page 112 and 113: HEK-Blue IL-1b Cells - IL-1b Repor
Page 114 and 115: HEK-Blue IL-33/IL-1b Cells - IL-33
Page 116 and 117: HEK-Blue CD40L Cells - CD40L Repor
Page 118 and 119: 5 ANTIBODIES & VACCINATION Antibodi
Page 120 and 121: Antibody generation using pFUSE-CHI
Page 122 and 123: Immunoglobulin A The mucosal surfac
Page 124 and 125: IgA Detection and Quantification In
Page 126 and 127: IgG Antibodies InvivoGen offers a s
Page 128 and 129: Mediators? 1. Leroux-Roels G., 2010
Page 130 and 131: Poly(I:C) Synthetic double-stranded
Page 132 and 133: pVAC - Induction of Neutralizing An
Page 134 and 135: CpG-Free DNA CpGs and the Immune Re
Page 136 and 137: pCpGfree-basic & pCpGfree-promoter
Page 138 and 139: pCpGfree-siRNA & pCpGfree-siRNADUO
Page 140 and 141: Custom-Made CpG-Free Genes InvivoGe
Page 143 and 144: RNA INTERFERENCE 7 psiRNA System I
Page 145 and 146: RNA INTERFERENCE 7 psiRNA-h7SK - 7S
Page 147 and 148: RNA INTERFERENCE 7 psiTEST System
Page 149 and 150: RNA INTERFERENCE 7 Ready-Made psiRN
Page 151 and 152: RNA INTERFERENCE 7 Custom-Made psiR
Page 153 and 154: INHIBITORS 8 Inhibitors PRODUCT DES
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INHIBITORS 8 Pattern Recognition Re
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INHIBITORS 8 Triptolide - NF-kB Inh
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INHIBITORS 8 Heat Shock Protein Inh
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9 CUSTOM SERVICES TLR/NOD Ligand Sc
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CUSTOM SERVICES 9 Custom Cloning :
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CUSTOM SERVICES 9 Prices 164 TERMS
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166 PRODUCT (QUANTITY) CAT. CODE PA
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A 17-AAG 17-AAGH2 17-AEP-GA 2-Amino
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- ODN 4084-F - ODN INH-1 - ODN INH-
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ARGENTINA Genbiotech S.R.L. Tel: +5
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