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Prom A-List - Human and Rodent Promoters Prom A-List is an expanding collection of native and composite promoters provided in the pDRIVE plasmid. Promoters are valuable tools to study the expression of a given gene both in vitro and in vivo. Now with pDRIVE, you can choose to express your gene of interest at high or low levels, ubiquitously or specifically, and in a constitutive or inducible manner. Features and Benefits An Expanding Collection of Promoters Each promoter is fully sequenced and its expression tested in different cell lines. Native or Composite Promoters • Native promoters, also called minimal promoters, consist of a single fragment from the 5’ region of a given gene. Each of them comprises a core promoter and its natural 5’UTR. In some cases, the 5’UTR contains an intron. • Composite promoters combine promoter elements of different origins (e.g. SV40 enhancer/AFP promoter) or were generated by assembling a distal enhancer with a minimal promoter of the same origin (e.g. CEA enhancer/promoter). Various Expression Patterns • Ubiquitous Promoters, strongly active in a wide range of cells, tissues and cell cycles. • Tissue Specific Promoters, active in a specific type of cells or tissues. • Tumor Specific Promoters, active specifically in tumor cells. Easy Subcloning of the Promoters In every pDRIVE, several unique restriction sites flank the promoter (a multiple cloning site at the 5’ end and Nco I or Bsp HI at the 3’ end) in order to excise the promoter easily. These restriction sites are compatible with many other enzymes, thus facilitating cloning. Other restriction sites may be introduced by performing PCR using primers containing the restriction sites of interest. Two Backbones with Different Reporter Genes In each pDRIVE, the promoter drives the expression of a reporter gene to facilitate the evaluation of its activity. Two different reporter genes are available in two different backbones: • LacZ in pDRIVE: pDRIVE plasmids contain the LacZ reporter gene which expression can be determined using chromogenic (see page 12), luminescent or histochemical detection. They carry the Sh ble gene conferring resistance to Zeocin downstream of the EM7 bacterial promoter. • SEAP in pDRIVE5-SEAP: pDRIVE5-SEAP plasmids feature an engineered secreted embryonic alkaline phosphatase (SEAP) reporter gene. SEAP expression levels can be assayed with chromogenic (see pages 13-15) or luminescent methods. Transient Expression in vitro pDRIVE plasmids are selectable with the antibiotic Zeocin in E. coli.They can be used for transient transfection experiments in mammalian cells in vitro or for expression studies in vivo. Related Products Custom-pDRIVE, page 162 Fast-Media ® Zeo, page 45 Contents and Storage www.invivogen.com/promoters Each pDRIVE plasmid is provided as lyophilized transformed E. coli strain. Transformed bacteria are shipped at room temperature. Store at -20°C. Transformed bacteria are stable for at least 1 year when properly stored. Each pDRIVE is provided with 4 pouches of E. coli Fast-Media ® Zeo (2 TB and 2 Agar). PRODUCT QTY CAT. CODE* pDRIVE-LacZ E. coli disk pdrive- pDRIVE-LacZ E. coli disk pdrive- pDRIVE5-SEAP E. coli disk pdrive5s- pDRIVE5-SEAP * See tables pages 40-41 E. coli disk pdrive5s- 39 MAMMALIAN EXPRESSION VECTORS 2
MAMMALIAN EXPRESSION VECTORS 2 b-Act Beta-Actin Ubiquitous Human 2387 bp pdrive-hbact pdrive5s-hbact EF-1a Elongation Factor 1 alpha Ubiquitous Chimpanzee 1365 bp pdrive-chef1 pdrive5s-chef1 Mouse 1315 bp pdrive-mef1 pdrive5s-mef1 Rat 1314 bp pdrive-ref1 pdrive5s-ref1 EGR1 Early Growth Response 1 Ubiquitous, inducible by radiation Human 1062 bp pdrive-hegr1 pdrive5s-hegr1 eIF4A1 Eukaryotic Initiation Factor 4A1 Ubiquitous Human 523 bp pdrive-heif4a1 pdrive5s-heif4a1 Mouse 482 bp pdrive-meif4a1 pdrive5s-meif4a1 FerH Ferritin Heavy Chain Ubiquitous Human 370 bp pdrive-hferh pdrive5s-hferh FerL Ferritin Light Chain Ubiquitous Human 429 bp pdrive-hferl pdrive5s-hferl GAPDH Glyceraldehyde 3-phosphate Dehydrogenase Ubiquitous Human 794 bp pdrive-hgapdh pdrive5s-hgapdh GRP78 Glucose-Regulated Protein 78 Ubiquitous, inducible by stress Human 531 bp pdrive-hgrp78 pdrive5s-hgrp78 Mouse 545 bp pdrive-mgrp78 pdrive5s-mgrp78 Rat 679 bp pdrive-rgrp78 pdrive5s-rgrp78 GRP94 Glucose-Regulated Protein 94 Ubiquitous, inducible by stress Human 701 bp pdrive-hgrp94 pdrive5s-hgrp94 Mouse 696 bp pdrive-mgrp94 pdrive5s-mgrp94 Rat 731 bp pdrive-rgrp94 pdrive5s-rgrp94 HSP70 Heat Shock Protein 70 Ubiquitous, inducible by heat Human 464 bp pdrive-hhsp70 pdrive5s-hhsp70 Mouse 1041 bp pdrive-mhsp70 pdrive5s-mhsp70 b-Kin Beta-Kinesin Ubiquitous Human 539 bp pdrive-hbkin pdrive5s-hbkin PGK1 Phosphoglycerate Kinase 1 Ubiquitous Mouse 1428 bp pdrive-mpgk pdrive5s-mpgk ROSA Rosa Ubiquitous Human 2815 bp pdrive-hrosa pdrive5s-hrosa Mouse 1926 bp pdrive-mrosa pdrive5s-mrosa Ubi B Ubiquitin B Ubiquitous Human 1092 bp pdrive-hubiquitinb pdrive5s-hubiquitinb Ubiquitous Composite Promoters b-Act/RU5’ Beta-Actin prom / HTLV 5’UTR Ubiquitous Human 1785 bp pdrive-hbactru5 pdrive-hbactru5 EF-1a/RU5’ Elongation Factor 1 prom / HTLV 5’UTR Ubiquitous Chimpanzee 672 bp pdrive-chef1ru5 pdrive5s-chef1ru5 Rat 630 bp pdrive-ref1ru5 pdrive5s-ref1ru5 FerH/RU5’ Ferritin H prom / HTLV 5’UTR Ubiquitous Human 457 bp pdrive-hferhru5 pdrive5s-hferhru5 SV40/FerH SV40 enh / Ferritin H prom / EF-1a 5’UTR Ubiquitous Human / mouse 1423 bp pdrive-sv40ferhef1 pdrive5s-sv40ferhef1 FerL/RU5’ Ferritin L prom / HTLV 5’UTR Ubiquitous Human 537 bp pdrive-hferlru5 pdrive5s-hferlru5 CMV/FerL CMV enh / Ferritin L prom / EF-1a 5’UTR Ubiquitous Human / chimp. 1666 bp pdrive-cmvferlef1 pdrive5s-cmvferlef1 Tissue-Specific Native Promoters B29 Immunoglobulin Beta B cells Human 1219 bp pdrive-hb29 pdrive5s-hb29 Mouse 1235 bp pdrive-mb29 pdrive5s-mb29 CD14 Monocyte Receptor for Bacterial LPS Monocytic cells Human 612 bp pdrive-hcd14 pdrive5s-hcd14 CD43 Leukosialin, Sialophorin Leukocytes and platelets Human 775 bp pdrive-hcd43 pdrive5s-hcd43 CD45 Leukocyte Common Antigen (LCA) Hematopoietic cells Human 856 bp pdrive-hcd45 pdrive5s-hcd45 CD68 Human Homolog of Macrosialin Macrophages Human 658 bp pdrive-hcd68 pdrive5s-hcd68 Mouse 811 bp pdrive-mcd68 pdrive5s-mcd68 Desmin Desmin Muscle Mouse 983 bp pdrive-mdesmin pdrive5s-mdesmin Elastase Elastase1 Pancreatic acinar cells Rat 264 bp pdrive-relastase pdrive5s-relastase Endoglin Endoglin Endothelial cells Human 886 bp pdrive-hendoglin pdrive5s-hendoglin FN Fibronectin Differentiating cells, healing tissues Human 1037 bp pdrive-hfibronectin pdrive5s-hfibronectin Flt-1 VEGF Receptor 1 Endothelial cells Human 786 bp pdrive-hflt1 pdrive5s-hflt1 GFAP Glial Fibrillary Acidic Protein Astrocytes Human 1675 bp pdrive-hgfap pdrive5s-hgfap Mouse 1679 bp pdrive-mgfap pdrive5s-mgfap Rat 1588 bp pdrive-rgfap pdrive5s-rgfap 40 PROMOTER GENE TISSUE DISTRIBUTION SPECIES SIZE Ubiquitous Native Promoters www.invivogen.com/promoters CAT. CODE (LacZ) CAT. CODE (SEAP)
Page 2 and 3: INNOVATION WITHIN REACH InvivoGen
Page 4 and 5: CONTENTS AT A GLANCE 1 CELL CULTURE
Page 6 and 7: 4 CYTOKINE SIGNALING TABLE of CONTE
Page 8 and 9: Mycoplasma Detection & Elimination
Page 10 and 11: PlasmoTest Contents - HEK-Blue -2
Page 12 and 13: Primocin - The Antimicrobial Shiel
Page 14 and 15: SEAP Reporter Gene System Secreted
Page 16 and 17: HEK-Blue Detection - Real-Time Det
Page 18 and 19: Blasticidin S Description Blasticid
Page 20 and 21: LyoVec New formulation LyoVec is
Page 22 and 23: Poly-Arginine-HA Tagged Reprogrammi
Page 24 and 25: LENTI-Smart - Lentiviral Vector Pr
Page 26 and 27: Lentiviral Vector Production and Ce
Page 28 and 29: Antibody generation using pFUSE-CHI
Page 30 and 31: Engineering Fc regions for altered
Page 32 and 33: pBROAD & pWHERE - Optimized Vector
Page 34 and 35: pSELECT-Tag - Expression of a GFP-,
Page 36 and 37: pVIVO - Expression of Two Genes of
Page 38 and 39: pORF Description pORF plasmids feat
Page 42 and 43: PROMOTER GENE TISSUE DISTRIBUTION S
Page 44 and 45: ChemiComp E. coli ChemiComp E. coli
Page 46 and 47: Fast-Media ® Preparation 1. Empty
Page 48 and 49: RIG-I-like Receptors 54 RLR & RLR-R
Page 50 and 51: Toll-Like Receptors Toll-Like Recep
Page 52 and 53: www.invivogen.com/innate-immunity 5
Page 54 and 55: IPAF and NAIP5 IPAF (NLRC4, CLAN/CA
Page 56 and 57: C-Type Lectin Receptors C-type lect
Page 58 and 59: pUNO pUNO-HA Toll-Like Receptors (T
Page 60 and 61: GENE NAME/ALIASES DESCRIPTION CAT.
Page 62 and 63: pDUO - Gene Associations Features a
Page 64 and 65: pZERO-TLR & pDeNy - Dominant Negati
Page 66 and 67: 293/TLR & 293/NOD Clones 293/TLR Cl
Page 68 and 69: Response of HEK-Blue hTLR2 cells t
Page 70 and 71: Ramos-Blue Cells - NF-kB/AP-1 Repo
Page 72 and 73: MEF Cells C3H/TLR4mut & C3H/WT MEFs
Page 74 and 75: Antibodies for Detection ANTIBODY D
Page 76 and 77: - NF-kB binding site: Nuclear facto
Page 78 and 79: PRODUCT ORIGIN/DESCRIPTION TLR2 Ago
Page 80 and 81: PRODUCT ORIGIN/DESCRIPTION TLR9 Ago
Page 82 and 83: PRODUCT ORIGIN/DESCRIPTION Dectin-1
Page 84 and 85: LPS-PG (Porphyromonas gingivalis) R
Page 86 and 87: • FLA-ST ultrapure was purified b
Page 88 and 89: 1. Zhao H. et al., 2004. Contributi
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1. Hornung V. et al., 2006. 5'-Trip
Page 92 and 93:
TLR/NOD Ligand Screening InvivoGen
Page 94 and 95:
Signal Transduction Inhibitors Invi
Page 96 and 97:
Pepinh-MYD - MyD88 inhibitory pepti
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1. Martinon F,. et al., 2002. The i
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Nigericin - NLRP3 Inflammasome Indu
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Autophagy is a pathway by which cyt
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4 CYTOKINE SIGNALING Cytokine Genes
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Blue Cytokine Reporter Cells Blue
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HEK-Blue IFN-a/b Cells - Human Typ
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HEK-Blue IL-6 Cells - IL6 Reporter
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HEK-Blue IL-1b Cells - IL-1b Repor
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HEK-Blue IL-33/IL-1b Cells - IL-33
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HEK-Blue CD40L Cells - CD40L Repor
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5 ANTIBODIES & VACCINATION Antibodi
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Antibody generation using pFUSE-CHI
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Immunoglobulin A The mucosal surfac
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IgA Detection and Quantification In
Page 126 and 127:
IgG Antibodies InvivoGen offers a s
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Mediators? 1. Leroux-Roels G., 2010
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Poly(I:C) Synthetic double-stranded
Page 132 and 133:
pVAC - Induction of Neutralizing An
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CpG-Free DNA CpGs and the Immune Re
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pCpGfree-basic & pCpGfree-promoter
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pCpGfree-siRNA & pCpGfree-siRNADUO
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Custom-Made CpG-Free Genes InvivoGe
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RNA INTERFERENCE 7 psiRNA System I
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RNA INTERFERENCE 7 psiRNA-h7SK - 7S
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RNA INTERFERENCE 7 psiTEST System
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RNA INTERFERENCE 7 Ready-Made psiRN
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RNA INTERFERENCE 7 Custom-Made psiR
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INHIBITORS 8 Inhibitors PRODUCT DES
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INHIBITORS 8 Pattern Recognition Re
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INHIBITORS 8 Triptolide - NF-kB Inh
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INHIBITORS 8 Heat Shock Protein Inh
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9 CUSTOM SERVICES TLR/NOD Ligand Sc
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CUSTOM SERVICES 9 Custom Cloning :
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CUSTOM SERVICES 9 Prices 164 TERMS
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166 PRODUCT (QUANTITY) CAT. CODE PA
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Page 171 and 172:
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A 17-AAG 17-AAGH2 17-AEP-GA 2-Amino
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- ODN 4084-F - ODN INH-1 - ODN INH-
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ARGENTINA Genbiotech S.R.L. Tel: +5
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