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HEK-Blue IL-33/IL-1b Cells - IL-33/IL-1b Reporter Cells NEW
➥ Monitor the IL-33/IL-1b-induced NF-kB and AP-1 pathways
➥ Detection of IL-33 and IL-1b(a)
Background
Interleukin-33 (IL-33) is a member of the IL-1 family, a group of cytokines
that play important roles in host defense, immune regulation and
inflammation. IL-33 mediates its biological effects through ST2 (also known
as IL1RL1), a receptor expressed on Th2 and mast cells. IL-33 and ST2 form
a complex with IL-1R accessory protein (IL-1RAcP), a signaling receptor
subunit that is also a member of the IL-1R complex. IL-33 signaling leads to
the activation of NF-kB and MAP kinases, and the production of Th2associated
cytokines.
Description
HEK-Blue IL-33/IL-1b cells are designed to detect bioactive IL-33 and
IL-1b (and IL-1a) by monitoring the activation of the NF-kB and AP-1
pathways. They were generated by stable transfection of HEK-Blue
IL-1b cells with the IL1RL1 gene. They express a SEAP reporter gene
under the control of the IFN-b minimal promoter fused to five NF-kB
and five AP-1 binding sites.
Stimulation of HEK-Blue IL-33/IL-1b cells with human IL-33 or IL-1b,
but not with IL-18 nor TNF-a, activates the NF-kB and AP-1 pathways
triggering the production of SEAP (figure 2). Levels of SEAP in the
supernatant can be easily determined with QUANTI-Blue .
Stimulation of HEK-Blue IL-33/IL-1b cells with recombinant human
IL-1b can be blocked by using the neutralizing antibody anti-hIL-1b-IgA.
• Detection range for human IL-33: 0.5 - 100 ng/ml
• Detection range for human IL-1b: 0.5 - 100 ng/ml
HEK-Blue IL-33/IL-1b cells are resistant to blasticidin, Zeocin and
hygromycin B.
Contents and Storage
HEK-Blue IL-33/IL-1b cells are grown in DMEM medium, 2mM
L-glutamine, 10% FBS supplemented with 30 μg/ml blasticidin, 100 μg/ml
Zeocin and 200 μg/ml HygroGold (ultrapure Hygromycin). Each vial
contains 5-7 x 10 6
cells and is supplied with 1 mg blasticidin, 10 mg Zeocin ,
10 mg HygroGold , 50 mg Normocin and 1 pouch of QUANTI-Blue Cells are shipped on dry ice.
Related Products
Figure 1: IL-33- and IL-1b--induced signaling pathways.
Figure 2: Stimulation of HEK-Blue IL-33/IL-1b cells by human IL-33, IL-1b, IL-18
and TNF-a was assessed by measuring the levels of SEAP using QUANTI-Blue .
Anti-hIL-1b-IgA , page 104 Blasticidin, page 17
Normocin , page 11 HygroGold , page 18
QUANTI-Blue , page 14 Zeocin , page 18 PRODUCT QUANTITY CAT. CODE
HEK-Blue IL-33/IL-1b Cells 5-7 x 10 6 cells hkb-il33
www.invivogen.com/cytokine-sensor-cells 113
CYTOKINE SIGNALING 4