scaricalo in formato PDF - labogen srl
scaricalo in formato PDF - labogen srl
scaricalo in formato PDF - labogen srl
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Reporter Cell L<strong>in</strong>es<br />
Eng<strong>in</strong>eered HEK293 Cells<br />
Cells that constitutively express a given functional TLR gene are valuable tools for many applications, such as the study of the mechanisms<br />
<strong>in</strong>volved <strong>in</strong> TLR recognition or signal<strong>in</strong>g, and the development of new potential therapeutic drugs. InvivoGen provides HEK293 cells stably<br />
express<strong>in</strong>g human or mur<strong>in</strong>e TLR or NOD genes.<br />
• 293/TLR & 293/NOD Clones<br />
• HEK-Blue TLR and HEK-Blue NOD Cells<br />
Immune Reporter Cells<br />
InvivoGen provides THP1-XBlue , Ramos-Blue and RAW-Blue Cells, derived from a human monocytic cell l<strong>in</strong>e, a human B lymphocyte and<br />
mur<strong>in</strong>e macrophages, respectively. They naturally express a large repertoire of different classes of pattern recognition receptors (PRRs), such<br />
as the TLRs, NLRs and RLRs and stably express an NF-kB-<strong>in</strong>ducible SEAP reporter gene to facilitate the study of these PRRs.<br />
• THP1-XBlue <br />
Cells<br />
• Ramos-Blue Cells<br />
• RAW-Blue <br />
Cells<br />
MEF Reporter Cell L<strong>in</strong>es<br />
InvivoGen provides mur<strong>in</strong>e embryonic fibroblasts (MEFs) isolated from various mouse stra<strong>in</strong>s and immortalized with the SV40 large antigen.<br />
They express a large repertoire of pattern recognition receptors. InvivoGen’s MEFs express an NF-kB-<strong>in</strong>ducible reporter (SEAP) system<br />
allow<strong>in</strong>g the convenient monitor<strong>in</strong>g of NF-kB activation follow<strong>in</strong>g TLR or RLR stimulation.<br />
• C3H/TLR4mut & C3H/WT MEFs<br />
• C57/WT MEFs<br />
Expression of TLR, NOD1/2 and RIG-I/MDA-5 mRNAs and their activity <strong>in</strong> InvivoGen’s Reporter Cell L<strong>in</strong>es*<br />
INNATE IMMUNITY 3+/- - +/- - +/- +/- - - - - +/- - - -<br />
64<br />
REPORTER CELL LINES TLR1 TLR2 TLR3 TLR4 TLR5 TLR6 TLR7 TLR8 TLR9 TLR10 NOD1 NOD2 RIG-I MDA-5<br />
HEK293-Null cells<br />
THP1-XBlue Cells<br />
Ramos-Blue Cells<br />
RAW-Blue Cells<br />
C3H MEFs<br />
C57 MEFs<br />
+ - + - + + - - - - + - - -<br />
+ + +/- + + + + + + + + + + +<br />
+ + - + + + - + - ND + ? ND ND<br />
+ + + + + + + + + + + - + +<br />
+/- +/- + - - +/- + - + + + - ND ND<br />
+ + + + +/- + + + + - +/- + + +<br />
+ + +/- + - + + ND + - - + ND ND<br />
+ + + + - + - - - - + + + +<br />
+ + + + - + - - - - ND ND ND ND<br />
+ + + + - + + + + - + + + +<br />
ND ND ND ND - ND ND ND ND - ND ND + +<br />
Rows with no background correspond to mRNA expression,rows with a grey background correspond to the activity<br />
B16-Blue IFN-a/b Cells<br />
InvivoGen provides B16-Blue IFN-a/b, a cell l<strong>in</strong>e that <strong>in</strong> addition to detect bioactive mur<strong>in</strong>e type I IFNs can also be used to study<br />
RIG-I/MDA-5 agonists, such as transfected poly(I:C) or 5’ppp-dsRNA (see page 106).<br />
www.<strong>in</strong>vivogen.com/reporter-cells
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