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CELL CULTURE & TRANSFECTION 1
QUANTI-Blue - Detection and Quantification of Alkaline Phosphatase
QUANTI-Blue is a detection medium developed to determine the activity of any alkaline phosphatase (AP) present in a biological sample.
QUANTI-Blue offers many advantages over the conventional SEAP Reporter Assay Kit based on the pNPP substrate:
➥ Shorter hands-on-time: no longer than 10 minutes
➥ Simpler preparation: just add water
➥ Allows high-throughput screening
➥ Higher saturation threshold
Key Features
Requires small samples of cell supernatants
Samples of 10 μl are sufficient.
Determine SEAP activity without disturbing cells
The same cell cultures can be repeatedly sampled for kinetic studies or
further experimentation.
Assay can be completed in 30 min
The enzymatic activity can be detected as early as 20 min after incubation
of the samples in QUANTI-Blue .
Highly sensitive for quantitative measurement
The assay allows to detect low and high levels of SEAP. No need to
perform multiple sample dilutions.
Wide dynamic range for accurate measurement
Higher saturation threshold than with pNPP resulting in more significant
differences between non or low SEAP expression and high SEAP
expression.
Contents and Storage
QUANTI-Blue is provided in a 5- or 10-pouch unit. Each pouch allows
the preparation of 100 ml of detection medium. Store at room
temperature. Pouches are stable 12 months at room temperature. After
preparation, product is stable 2 weeks at 4°C and 2 months at -20°C.
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PRODUCT QUANTITY CAT. CODE
QUANTI-Blue 5 pouches (5 x 100 ml)
10 pouches (10 x 100 ml)
* Bulk quantities readily available
rep-qb1
rep-qb2
www.invivogen.com/reporter-detection
QUANTI-Blue Procedure