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MAMMALIAN EXPRESSION VECTORS 2
pVITRO - Expression of Two Genes of Interest in vitro
pVITRO is a family of plasmids developed mainly for in vitro studies. They allow the ubiquitous and constitutive co-expression of two genes of
interest. pVITRO plasmids can be stably transfected in mammalian cells and the genes of interest are expressed at high levels. Each pVITRO
plasmid is available with either two multiple cloning sites or two reporter genes.
Features and Benefits
Gene Combinations in a Single Plasmid
Co-expression of two or more genes from a single vector is more efficient
and convenient than using two separate vectors. pVITRO are multigenic
plasmids that contain two distinct transcription units (TU).
Strong and Constitutive Expression of Two Transgenes
Each pVITRO plasmid features two constitutive promoters that drive high
levels of expression from two separate TU in a large number of
mammalian cell lines. Transcriptional interference is minimized by using
promoters of different origins or promoters that are coordinately
activated (i.e. ferritin promoters), and strong polyadenylation signals
(polyA).
• pVITRO1 plasmids carry two elongation factor 1 alpha (EF-1a)
promoters, from rat and mouse origins combined to the CMV and SV40
enhancers respectively.
• pVITRO2 plasmids contain two human ferritin composite promoters,
FerH (heavy chain) and FerL (light chain), combined to the SV40 and
CMV enhancers respectively. To eliminate the iron regulation, their 5’UTRs
have been replaced by the 5’UTR of the mouse and chimpanzee EF-1a
genes.
Single Selection Marker for E. coli and Mammalian Cells
pVITRO plasmids are available with different selectable markers that are
active both in E. coli and mammalian cells: bsr (blasticidin resistance), hph
(hygromycin resistance), neo (kanamycin/G418 resistance). In bacteria, the
resistance gene is expressed from the E. coli EM7 promoter. In mammalian
cells, it is transcribed from the promoter located 3’ of the Ori, as a
polycistronic mRNA and translated through the IRES of the Foot and
Mouth Disease Virus.
Available with Two MCS or Two Reporter Genes
pVITRO-mcs plasmids contain two multiple cloning sites (MCS) for the
convenient cloning of cDNAs.
pVITRO-GFP/LacZ express the GFP and LacZ reporter genes, and can
be used as controls. As the reporter genes are flanked by unique
restriction sites, they can be used for the cloning of open reading frames
that can be chosen from InvivoGen’s Gene A-List (see page 36 or visit
our website www.invivogen.com/orfs).
Contents and Storage
Each pVITRO plasmid is provided as 20 μg of lyophilized DNA. Product
is shipped at room temperature and should be stored at -20°C. Plasmid
is stable up to one year when properly stored.
Each pVITRO is provided with 4 pouches of the appropriate E. coli Fast-
Media ® (2 TB and 2 Agar).
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Related Products
Blasticidin, page 17 G418, page 17
HygroGold , see page 18 Puromycin, page 17
Zeocin , page 18 E. coli Fast-Media ® , pages 44-45
MCS1
or
GFP
MCS1
or
GFP
www.invivogen.com/gene-expression
MCS 1 5’- Bsp EI, Bst 1107I, Bam HI, Bsi WI, Avr II -3’
MCS 2 5’- Age I, Eco RV, Bgl II, Bsr GI, Nhe I -3’
MCS2
or
LacZ
MCS2
or
LacZ
MCS 1 5’- Age I, Eco RV, Bam HI, Mlu I, Cla I, Sal I, Avr II -3’
MCS 2 5’- Sgr AI, Fsp I, Bgl II, Eco RI, Bst BI, Xho I, Nhe I-3’
PRODUCT QTY CAT. CODE
pVITRO1-blasti-mcs 20 μg pvitro1-bmcs
pVITRO1-blasti-GFP/LacZ 20 μg pvitro1-bgfplacz
pVITRO1-hygro-mcs 20 μg pvitro1-mcs
pVITRO1-hygro-GFP/LacZ 20 μg pvitro1-gfplacz
pVITRO1-neo-mcs 20 μg pvitro1-nmcs
pVITRO1-neo-GFP/LacZ 20 μg pvitro1-ngfplacz
pVITRO2-blasti-mcs 20 μg pvitro2-bmcs
pVITRO2-blasti-GFP/LacZ 20 μg pvitro2-bgfplacz
pVITRO2-hygro-mcs 20 μg pvitro2-mcs
pVITRO2-hygro-GFP/LacZ 20 μg pvitro2-gfplacz
pVITRO2-neo-mcs 20 μg pvitro2-nmcs
pVITRO2-neo-GFP/LacZ 20 μg pvitro2-ngfplacz