scaricalo in formato PDF - labogen srl

Recommendations

Info

pCpGfree-basic & pCpGfree-promoter NEW Promoter CpG Methylation Study Methylation of CpG dinucleotides within the promoter region of genes is often associated with transcriptional silencing. This epigenetic event plays an important role in the regulation of gene activity in normal and cancer cells. InvivoGen provides pCpGfree-basic and pCpGfree-promoter, two SEAP reporter plasmids completely devoid of CpG dinucleotides, that allow to study the effect of promoter CpG methylation in transfection assays. The lack of CpGs within the plasmid backbone limits in vitro CpG methylation to the CpG dinucleotides present in the inserted promoter fragment. Thus, the pCpGfree-basic and pCpGfree-promoter plasmids represent useful tools to analyze the effect of DNA methylation on CpG-containing promoters. Description The pCpGfree-basic and pCpGfree-promoter plasmids derive from the pCpGfree-mSEAP plasmid by deletion of the enhancer/promoter region or the enhancer sequence alone, respectively. pCpGfree-basic The pCpGfree-basic plasmid lacks the entire promoter region (i. e. murine CMV enhancer and human EF-1a promoter). It contains a multiple cloning site upstream of the murine secreted embryonic alkaline phosphatase (mSEAP) reporter gene. Expression of mSEAP in cells transfected with this plasmid depends on the insertion of a functional promoter or enhancer/promoter cassette upstream from the mSEAP gene. Thus, pCpGfree-basic allows to study the effect of CpG methylation on a promoter, alone or combined with enhancer elements. pCpGfree-promoter The pCpGfree-promoter plasmid contains the human EF-1a promoter and a multiple cloning site in place of the murine CMV enhancer. It is specifically designed to analyze the effect of methylation on CpG residues present in enhancer elements. Procedure 1- Insert promoter and/or enhancer fragment into pCpGfree-basic or pCpGfree-promoter. 2- Treat recombinant plasmid with CpG methylase (Sss I) or site specific-methylase (Hha I, Hpa II). 3- Transiently transfect cells with methylase-treated or untreated plasmid. 4- Analyze promoter CpG methylation by determining mSEAP expression using QUANTI-Blue , a SEAP detection medium. Contents and Storage pCpGfree-basic and pCpGfree-promoter plasmids are provided as 20 μg of lyophilized DNA with a paper disk of lyophilized E. coli GT115 strain. Products are shipped at room temperature and should be stored at -20°C. Zeo R MAR EF1 prom Ori EM2K MCS SI126 pCpGfree-promoter MAR pAn mSEAP PRODUCT QUANTITY CAT. CODE pCpGfree-basic NEW 20 μg pcpgf-bas pCpGfree-promoter NEW 20 μg pcpgf-prom SdaI Bsp120I AvrII NsiI Ppu10I ScaI BamHI SpeI HindIII BsrGI AflIII BbsI SdaI Bsp120I AvrII NsiI Ppu10I ScaI BamHI SpeI HindIII BsrGI AflIII BbsI Important Licensing Information: These products are covered by a Limited Use License (See Terms and Conditions on page 164). By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. www.invivogen.com/cpgfree-plasmid 135 CpG-FREE DNA 6
CpG-FREE DNA 6 pCpGfree-vitro - Selectable CpG-free Plasmids Description CpG-free Plasmid Backbone pCpGfree-vitro is a family of expression vectors completely devoid of CpG dinucleotides that are selectable in mammalian cells. Similarly to the other pCpGfree plasmids, all the elements required for replication and selection of the plasmids in bacteria, and gene expression in mammalian cells have been modified to remove all CpG dinucleotides. A Choice of Different Backbones To better suit your needs, the pCpGfree-vitro plasmid comes with a choice of selection: blasticidin, hygromycin or kanamycin/G418. Each pCpGfree-vitro is available with a CpG-free allele of the LacZ gene or a multiple cloning site (MCS). This MCS contains several commonly used restriction sites, for convenient cloning of a CpG-free gene, such as the genes provided in the pSELECT plasmid (see page 138) or any open reading frame or cDNA. Applications Study the immunostimulatory effect of CpG motifs pCpGfree-vitro plasmids represent innovative tools to study the effects of CpG dinucleotides in a number of applications. DNA vaccination exploits the immunostimulatory character of certain CpG motifs to prime and boost the immune response. However, these immunostimulatory CpG motifs are antagonized by CpG dinucleotides in certain distinct base contexts, termed neutralizing CpG motifs. Both types of CpG motifs are usually present in plasmidic DNA, and therefore may lead to an unfavorable immune response. pCpGfree-vitro is the ideal tool to overcome this problem, and may be used to study the effects of these two types of CpG motifs by adding them in different configurations to the pCpGfree-vitro backbone. Study CpG methylation CpG dinucleotides are key elements in a number of cellular functions associated with chromatin. Several large multisubunit complexes, consisting of methyl-CpG binding (MBD) proteins and histone deacetylases, have been implicated in the regulation of chromatin dynamics. These complexes are recruited to methylated CpG dinucleotides by DNA methyl transferases (DNMTs) and induce chromatin remodeling. However the specific roles of these complexes are still to be explored. Due to the absence of CpG dinucleotides within its backbone, pCpGfree-vitro is not the target of DNMTs and thus MBD proteins. Therefore, it provides a useful model to study the other proteins involved in these complexes, in particular the histone deacetylases. It can also be used to analyze the effects of CpG methylation on the regulation and duration of gene expression. Contents and Storage Each pCpGfree-vitro plasmid is provided as 20 μg of lyophilized DNA with a paper disk of lyophilized E. coli GT115 strain. Products are shipped at room temperature and should be stored at -20°C. Selections Available in pCpGfree-vitro Plasmids - Blasticidin - Hygromycin - Neomycin (G418) PRODUCT QUANTITY CAT. CODE pCpGfree-vitroBmcs 20 μg pcpgvtb-mcsg2 pCpGfree-vitroBLacZ 20 μg pcpgvtb-lz pCpGfree-vitroHmcs 20 μg pcpgvth-mcsg2 pCpGfree-vitroHLacZ 20 μg pcpgvth-lz pCpGfree-vitroNmcs 20 μg pcpgvtn-mcsg2 pCpGfree-vitroNLacZ 20 μg pcpgvtn-lz Related Products Important Licensing Information: These products are covered by a Limited Use License (See Terms and Conditions on page 164). By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. 136 www.invivogen.com/cpgfree-plasmid Blasticidin, page 17 Hygromycin B, page 18 G418 Sulfate, page 17 LyoComp GT115, page 43 MCS BsrGI ScaI BglII ApaLI Bsp120I NcoI NheI MscI
Page 2 and 3:
INNOVATION WITHIN REACH InvivoGen
Page 4 and 5:
CONTENTS AT A GLANCE 1 CELL CULTURE
Page 6 and 7:
4 CYTOKINE SIGNALING TABLE of CONTE
Page 8 and 9:
Mycoplasma Detection & Elimination
Page 10 and 11:
PlasmoTest Contents - HEK-Blue -2
Page 12 and 13:
Primocin - The Antimicrobial Shiel
Page 14 and 15:
SEAP Reporter Gene System Secreted
Page 16 and 17:
HEK-Blue Detection - Real-Time Det
Page 18 and 19:
Blasticidin S Description Blasticid
Page 20 and 21:
LyoVec New formulation LyoVec is
Page 22 and 23:
Poly-Arginine-HA Tagged Reprogrammi
Page 24 and 25:
LENTI-Smart - Lentiviral Vector Pr
Page 26 and 27:
Lentiviral Vector Production and Ce
Page 28 and 29:
Antibody generation using pFUSE-CHI
Page 30 and 31:
Engineering Fc regions for altered
Page 32 and 33:
pBROAD & pWHERE - Optimized Vector
Page 34 and 35:
pSELECT-Tag - Expression of a GFP-,
Page 36 and 37:
pVIVO - Expression of Two Genes of
Page 38 and 39:
pORF Description pORF plasmids feat
Page 40 and 41:
Prom A-List - Human and Rodent Pro
Page 42 and 43:
PROMOTER GENE TISSUE DISTRIBUTION S
Page 44 and 45:
ChemiComp E. coli ChemiComp E. coli
Page 46 and 47:
Fast-Media ® Preparation 1. Empty
Page 48 and 49:
RIG-I-like Receptors 54 RLR & RLR-R
Page 50 and 51:
Toll-Like Receptors Toll-Like Recep
Page 52 and 53:
www.invivogen.com/innate-immunity 5
Page 54 and 55:
IPAF and NAIP5 IPAF (NLRC4, CLAN/CA
Page 56 and 57:
C-Type Lectin Receptors C-type lect
Page 58 and 59:
pUNO pUNO-HA Toll-Like Receptors (T
Page 60 and 61:
GENE NAME/ALIASES DESCRIPTION CAT.
Page 62 and 63:
pDUO - Gene Associations Features a
Page 64 and 65:
pZERO-TLR & pDeNy - Dominant Negati
Page 66 and 67:
293/TLR & 293/NOD Clones 293/TLR Cl
Page 68 and 69:
Response of HEK-Blue hTLR2 cells t
Page 70 and 71:
Ramos-Blue Cells - NF-kB/AP-1 Repo
Page 72 and 73:
MEF Cells C3H/TLR4mut & C3H/WT MEFs
Page 74 and 75:
Antibodies for Detection ANTIBODY D
Page 76 and 77:
- NF-kB binding site: Nuclear facto
Page 78 and 79:
PRODUCT ORIGIN/DESCRIPTION TLR2 Ago
Page 80 and 81:
PRODUCT ORIGIN/DESCRIPTION TLR9 Ago
Page 82 and 83:
PRODUCT ORIGIN/DESCRIPTION Dectin-1
Page 84 and 85:
LPS-PG (Porphyromonas gingivalis) R
Page 86 and 87: • FLA-ST ultrapure was purified b
Page 88 and 89: 1. Zhao H. et al., 2004. Contributi
Page 90 and 91: 1. Hornung V. et al., 2006. 5'-Trip
Page 92 and 93: TLR/NOD Ligand Screening InvivoGen
Page 94 and 95: Signal Transduction Inhibitors Invi
Page 96 and 97: Pepinh-MYD - MyD88 inhibitory pepti
Page 98 and 99: 1. Martinon F,. et al., 2002. The i
Page 100 and 101: Nigericin - NLRP3 Inflammasome Indu
Page 102 and 103: Autophagy is a pathway by which cyt
Page 104 and 105: 4 CYTOKINE SIGNALING Cytokine Genes
Page 106 and 107: Blue Cytokine Reporter Cells Blue
Page 108 and 109: HEK-Blue IFN-a/b Cells - Human Typ
Page 110 and 111: HEK-Blue IL-6 Cells - IL6 Reporter
Page 112 and 113: HEK-Blue IL-1b Cells - IL-1b Repor
Page 114 and 115: HEK-Blue IL-33/IL-1b Cells - IL-33
Page 116 and 117: HEK-Blue CD40L Cells - CD40L Repor
Page 118 and 119: 5 ANTIBODIES & VACCINATION Antibodi
Page 120 and 121: Antibody generation using pFUSE-CHI
Page 122 and 123: Immunoglobulin A The mucosal surfac
Page 124 and 125: IgA Detection and Quantification In
Page 126 and 127: IgG Antibodies InvivoGen offers a s
Page 128 and 129: Mediators? 1. Leroux-Roels G., 2010
Page 130 and 131: Poly(I:C) Synthetic double-stranded
Page 132 and 133: pVAC - Induction of Neutralizing An
Page 134 and 135: CpG-Free DNA CpGs and the Immune Re
Page 138 and 139: pCpGfree-siRNA & pCpGfree-siRNADUO
Page 140 and 141: Custom-Made CpG-Free Genes InvivoGe
Page 143 and 144: RNA INTERFERENCE 7 psiRNA System I
Page 145 and 146: RNA INTERFERENCE 7 psiRNA-h7SK - 7S
Page 147 and 148: RNA INTERFERENCE 7 psiTEST System
Page 149 and 150: RNA INTERFERENCE 7 Ready-Made psiRN
Page 151 and 152: RNA INTERFERENCE 7 Custom-Made psiR
Page 153 and 154: INHIBITORS 8 Inhibitors PRODUCT DES
Page 155 and 156: INHIBITORS 8 Pattern Recognition Re
Page 157 and 158: INHIBITORS 8 Triptolide - NF-kB Inh
Page 159 and 160: INHIBITORS 8 Heat Shock Protein Inh
Page 161 and 162: 9 CUSTOM SERVICES TLR/NOD Ligand Sc
Page 163 and 164: CUSTOM SERVICES 9 Custom Cloning :
Page 165 and 166: CUSTOM SERVICES 9 Prices 164 TERMS
Page 167 and 168: 166 PRODUCT (QUANTITY) CAT. CODE PA
Page 173 and 174: A 17-AAG 17-AAGH2 17-AEP-GA 2-Amino
Page 175 and 176: - ODN 4084-F - ODN INH-1 - ODN INH-
Page 177 and 178: ARGENTINA Genbiotech S.R.L. Tel: +5
show all

scaricalo in formato PDF - labogen srl

Create successful ePaper yourself

Delete template?

Save as template?