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Response of HEK-Blue hTLR2 cells to TLR2 agonists.
Cells were incubated in HEK-Blue Detection medium
and stimulated with 0.1 ng/ml FSL-1 (TLR2/6), 0.1 ng/ml
Pam3CSK4(TLR1/2), 10 7 cells/ml HKLM, 1 μg/ml LTA-
SA, 1 ng/ml LPS-PG or 1 μg/ml PGN-BS. After 24h
incubation, the levels of NF-kB-induced SEAP were
determined by reading the OD at 655 nm.
Response of HEK-Blue hTLR5 cells to TLR5 agonists.
Cells were incubated in HEK-Blue Detection medium
and stimulated with 100 ng/ml FLA-BS, 10 ng/ml
FLA-ST ultrapure or 10 ng/ml recFLA-ST. After 24h
incubation, the levels of NF-kB-induced SEAP were
determined by reading the OD at 655 nm.
Response of HEK-Blue hTLR9 cells to TLR9 agonists.
Cells were stimulated with 1 μg/ml ODN2006 (type B),
1 μg/ml ODN2216 (type A) or 5 μg/ml E. coli ssDNA.
After 24h incubation, the levels of NF-kB-induced SEAP
were determined using QUANTI-Blue by reading the
OD at 655 nm.
Response of HEK-Blue hTLR3 cells to TLR3 agonists.
Cells were stimulated with 100 ng/ml poly(I:C)
(HMW), 100 ng/ml poly(I:C)-LMW or 1 μg/ml
poly(A:U). After 24h incubation, the levels of NF-kBinduced
SEAP were determined using QUANTI-Blue
by reading the OD at 655 nm.
Response of HEK-Blue hTLR7 cells to TLR7/8
agonists. Cells were incubated in HEK-Blue Detection
medium and stimulated with 100 ng/ml CL264, 5 μg/ml
imiquimod, 1 μg/ml gardiquimod or 100 ng/ml R848.
After 24h incubation, the levels of NF-kB-induced SEAP
were determined by reading the OD at 655 nm.
Response of HEK-Blue hNOD1 cells to NOD1/2
agonists. Cells were incubated in HEK-Blue Detection
medium and stimulated with 1 μg/ml iE-DAP, 100 ng/ml
C12-iE-DAP, 1 μg/ml TRI-DAP or 1 μg/ml M-TriDAP.
After 24h incubation, the levels of NF-kB-induced SEAP
were determined by reading the OD at 655 nm.
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Response of HEK-Blue hTLR4 cells to TLR4 agonists.
Cells were incubated in HEK-Blue Detection medium
and stimulated with 10 ng/ml LPS-EB ultrapure, 10
ng/ml LPS-EK ultrapure, 100 ng/ml MPLA or 100 ng/ml
MPLAs. After 24h incubation, the levels of NF-kBinduced
SEAP were determined by reading the OD at
655 nm.
Response of HEK-Blue hTLR8 cells to TLR7/8
agonists. Cells were incubated in HEK-Blue Detection
medium and stimulated with 5 μg/ml ssRNA40, 1 μg/ml
CL075 or1 μg/ml R848. After 24h incubation, the levels
of NF-kB-induced SEAP were determined by reading
the OD at 655 nm.
Response of HEK-Blue hNOD2 cells to NOD1/2
agonists. Cells were incubated in HEK-Blue Detection
medium and stimulated with 100 ng/ml MDP, 10 ng/ml
L18-MDP 100 ng/ml murabutide or 100 ng/ml M-TriDAP.
After 24h incubation, the levels of NF-kB-induced SEAP
were determined by reading the OD at 655 nm.
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