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MAMMALIAN EXPRESSION VECTORS 2
pMONO & pSELECT - Expression of One Gene of Interest
pMONO & pSELECT plasmids are specifically designed for strong and constitutive expression of a gene of interest in a wide variety of mammalian
cell lines. They allow the selection of stable transfectants and offer a choice of selectable markers for both E. coli and mammalian cells.
• pMONO plasmids contain a unique transcription unit that drives the expression of the gene of interest and the selectable marker through
an internal ribosome entry site (IRES). This dual gene expression system ensures that stable clones express the gene of interest.
• pSELECT plasmids contain two transcription units, the first drives the expression of the gene of interest and the second drives the
expression of the resistance gene.
Features and Benefits
Strong and Ubiquitous Expression of the Transgene
- pMONO plasmids feature the SV40/FerH/mEF-1a promoter composed
of the ferritin heavy chain (FerH) core promoter fused at its 5’ end to
the SV40 enhancer, and at its 3’ end to the intron-containing 5’UTR of
the mouse elongation factor 1 alpha gene.
- pSELECT plasmids feature the strong EF-1a/HTLV composite promoter
that combines the elongation factor 1 alpha core promoter and the
5’untranslated region of the Human T-cell Leukemia Virus.
Variety of Selection Options
- pMONO plasmids are selectable with blasticidin, hygromycin,
kanamycin/G418 and Zeocin . All resistance genes are cloned
downstream of the IRES from the Foot and Mouth Disease Virus (FMDV),
eliminating the need of a second mammalian promoter. The FMDV IRES
is in tandem with the constitutive bacterial EM7 promoter conferring
resistance both in E. coli and mammalian cells.
- pSELECT plasmids are selectable with blasticidin, hygromycin,
kanamycin/G418, puromycin and Zeocin . These selectable markers are
driven by the CMV promoter in tandem with the bacterial EM7 promoter
for selection in both E. coli and mammalian cells. pSELECT is also available
with the GFPzeo fusion gene that combines the GFP reporter gene and
the Zeocin resistance gene.
Multiple Cloning Site or Reporter Gene
- pMONO plasmids carry a multiple cloning site (MCS) downstream of
the SV40/FerH/mEF-1a promoter for convenient cloning of a gene of
interest. pMONO plasmids are also available with a GFP allele and can
be used as control vectors.
- pSELECT plasmids contain an MCS downstream of the EF-1a/HTLV
promoter or the LacZ reporter gene as a control.
Contents and Storage
pMONO and pSELECT plasmids are provided as 20 μg of lyophilized DNA.
Product is shipped at room temperature and should be stored at -20°C.
Plasmid is stable up to one year when properly stored.
pMONO and pSELECT plasmids are provided with 4 pouches of E. coli Fast-
Media ® containing the appropriate selective antibiotic (2 TB and 2 Agar).
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Related Products
Blasticidin, page 17 HygroGold , see page 18
Puromycin, page 17 Zeocin , page 18
Fast-Media ® , pages 44-45
PRODUCT QTY
www.invivogen.com/gene-expression
CAT. CODE
(MCS)
MCS
or
GFP
CAT. CODE
(GFP)
pMONO-blasti-mcs 20 μg pmonob-mcs pmonob-gfp
pMONO-hygro-mcs 20 μg pmonoh-mcs pmonoh-gfp
pMONO-neo-mcs 20 μg pmonon-mcs pmonon-gfp
pMONO-zeo-mcs 20 μg pmonoz-mcs pmonoz-gfp
PRODUCT QTY
CAT. CODE
(MCS)
MCS
or
LacZ
CAT. CODE
(LacZ)
pSELECT-blasti 20 μg psetb-mcs psetb-lacz
pSELECT-hygro 20 μg pseth-mcs pseth-lacz
pSELECT-neo 20 μg psetn-mcs psetn-lacz
pSELECT-puro 20 μg psetp-mcs psetp-lacz
pSELECT-zeo 20 μg psetz-mcs psetz-lacz
pSELECT-GFPzeo 20 μg psetgz-mcs psetgz-lacz