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INNATE IMMUNITY 3
pUNO - Native Genes
Features and Benefits
Numerous genes encoding pattern recognition receptors (PRRs), co-receptors, adaptors,
signaling effectors and signaling inhibitors are available in the pUNO plasmid. The genes are
cloned from the ATG to the Stop codon, excluding introns and untranslated regions. All
genes are fully sequenced, the sequences are available online at www.invivogen.com or can
be emailed upon request.
PRR and related genes are cloned under the control of the strong and ubiquitous mammalian
promoter, EF1a/HTLV. This composite promoter is comprised of the elongation factor 1
alpha (EF-1a) core promoter and the R-U5’ of the human T cell leukemia virus (HTLV).
pUNO plasmids can be used directly for in vitro or in vivo transfection experiments. They are
selectable with blasticidin in both E. coli and mammalian cells. To facilitate the excision and
subcloning of the gene of interest into another vector, each gene is flanked by unique
restriction sites that are compatible with many others.
Some genes are provided in the pUNO2 (Zeocin -resistant) or pUNO3 (hygromycinresistant)
backbone
Contents and Storage
Each pUNO plasmid is provided as a lyophilized transformed E. coli strain on a paper disk.
Transformed strains are shipped at room temperature and should be stored at -20ºC.
Lyophilized E. coli cells are stable for at least 1 year when properly stored. Each plasmid is
provided with 4 pouches of E. coli Fast-Media ® Blas (2 TB and 2 Agar).
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Toll-Like Receptors (TLRs)
TLR genes from different species are available:
- human
- mouse
- pig
- bovine
Human TLR genes are available in two different plasmid backbones:
- pUNO/pUNO1 selectable with blasticidin
- pUNO3, selectable with hygromycin
GENE NAME DESCRIPTION
TOLL-Like Receptors (TLRs)
CAT. CODE
(human)
CAT. CODE
(human, hygro)
CAT. CODE
(mouse)
TLR1 Toll-like Receptor 1 puno-htlr1 puno3-htlr1 puno-mtlr1 puno1-ptlr1 NEW -
TLR2 Toll-like Receptor 2 puno-htlr2 puno3-htlr2 puno-mtlr2 puno1-ptlr2 NEW puno1-btlr2 NEW
TLR3 Toll-like Receptor 3 puno-htlr3 puno3-htlr3 puno-mtlr3 -
TLR4 Toll-like Receptor 4 puno1-htlr4a puno3-htlr4a puno-mtlr4 -
TLR5 Toll-like Receptor 5 puno-htlr5 puno3-htlr5 puno-mtlr5 puno1-ptlr5 NEW puno1-btlr5 NEW
TLR6 Toll-like Receptor 6 puno-htlr6 puno3-htlr6 puno-mtlr6 puno1-ptlr6 NEW puno1-btlr6 NEW
TLR7 Toll-like Receptor 7 puno-htlr7 puno3-htlr7 puno-mtlr7 puno1-ptlr7 NEW puno1-btlr7 NEW
TLR8 Toll-like Receptor 8 puno1-htlr8b puno3-htlr8b puno-mtlr8 -
TLR9 Toll-like Receptor 9 puno1-htlr9a puno3-htlr9a puno-mtlr9 puno1-ptlr9 NEW puno1-btlr9 NEW
TLR10 Toll-like Receptor 10 puno-htlr10 puno3-htlr10 - puno1-ptlr10 NEW
TLR11 Toll-like Receptor 11 - - puno-mtlr11t -
TLR11/12 Toll-like Receptor 11/12 - - puno-mtlr11z -
TLR13 Toll-like Receptor 13 - - puno-mtlr13 -
www.invivogen.com/innate-immunity-puno
Related Products
Blasticidin, page 17
Hygromycin B, HygroGold , page 18
Fast-Media ® Blas, page 45
Fast-Media ® Hygro, page 45
CAT. CODE
(pig)
CAT. CODE
(bovine)