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INNATE IMMUNITY 3
pUNO-HA - TLR-HA Genes
Features and Benefits
pUNO-HA is a family of expression vectors featuring HA-tagged TLR and IRF (Interferon
Regulatory Factor) genes. The genes have been fused at the 3’end to the influenza
hemaglutinine (HA) tag. This short sequence (YPYDVPDYA) encodes a peptide which is the
epitope of a very efficient and specific monoclonal antibody. The use of HA-tagged TLR genes
provides a simple and convenient method to detect the expression of the TLR genes by
Western blot. All TLR genes can be detected using the same primary antibody, the HA tag
monoclonal antibody (see page 73).
pUNO-HA plasmids are selectable with blasticidin in both E. coli and mammalian cells.
pUNO-TLR-GFP - TLR-GFP Fusion Genes
TLR-GFP fusion proteins can be used to study the localization of the TLRs. Transfected
cells can be analyzed for GFP expression by flow cytometry and by Western-blotting
using GFP antibodies.
Features and Benefits
TLR-GFP fusion genes were generated by fusing the GFP gene to the C terminus of various
human TLR genes (TLR1 to TLR6). The TLR-GFP fusion genes are cloned in the pUNO
plasmid under the control of the strong and ubiquitous mammalian promoter EF1a/HTLV.
The pUNO plasmid is selectable with blasticidin in both E. coli and mammalian cells.
All TLR::GFP fusion genes have been fully sequenced, their fluorescence confirmed and their
function tested in HEK293 cells coexpressing an NF-kB reporter plasmid and stimulated with
the appropriate ligand.
Contents and Storage
62
GENE PLASMID QTY
CAT. CODE
(human)
CAT. CODE
(mouse)
TLR1-HA pUNO-TLR1-HA E. coli disk punoha-htlr1 punoha-mtlr1
TLR2-HA pUNO-TLR2-HA E. coli disk punoha-htlr2 punoha-mtlr2
TLR3-HA pUNO-TLR3-HA E. coli disk punoha-htlr3 punoha-mtlr3
TLR4-HA pUNO-TLR4-HA E. coli disk punoha-htlr4a punoha-mtlr4
TLR5-HA pUNO-TLR5-HA E. coli disk punoha-htlr5 punoha-mtlr5
TLR6-HA pUNO-TLR6-HA E. coli disk punoha-htlr6 punoha-mtlr6
TLR7-HA pUNO-TLR7-HA E. coli disk punoha-htlr7 punoha-mtlr7
TLR8-HA pUNO-TLR8-HA E. coli disk punoha-htlr8a punoha-mtlr8
TLR9-HA pUNO-TLR8-HA E. coli disk punoha-htlr9a punoha-mtlr9
TLR10-HA pUNO-TLR10-HA E. coli disk punoha-htlr10 -
TLR11-HA pUNO-TLR11-HA E. coli disk - punoha-mtlr11
Each pUNO-TLR-GFP plasmid is provided as a lyophilized transformed
E. coli strain on a paper disk. Transformed strains are shipped at room
temperature and should be stored at -20ºC. Lyophilized E. coli cells are
stable for at least 1 year when properly stored.
Each plasmid is provided with 4 pouches of E. coli Fast-Media ® Blas (2 TB
and 2 Agar, see pages 44-45).
www.invivogen.com/innate-immunity
Contents and Storage
Each pUNO-HA plasmid is provided as a lyophilized
transformed E. coli strain on a paper disk. Transformed
strains are shipped at room temperature and should
be stored at -20ºC. Lyophilized E. coli cells are stable
for at least 1 year when properly stored.
Each plasmid is provided with 4 pouches of E.coli
Fast-Media ® Blas (2 TB and 2 Agar). For more
information about Fast-Media ® , see pages 44-45.
GENE PLASMID QTY CAT. CODE
hTLR1-GFP pUNO-hTLR1-GFP E. coli disk phtlr1-gfp
hTLR2-GFP pUNO-hTLR2-GFP E. coli disk phtlr2-gfp
hTLR3-GFP pUNO-hTLR3-GFP E. coli disk phtlr3-gfp
hTLR4-GFP pUNO-hTLR4-GFP E. coli disk phtlr4-gfp
hTLR5-GFP pUNO-hTLR5-GFP E. coli disk phtlr5-gfp
hTLR6-GFP pUNO-hTLR6-GFP E. coli disk phtlr6-gfp