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MAMMALIAN EXPRESSION VECTORS 2
pFUSE-Fc - Fc-Fusion Proteins
pFUSE-Fc is a family of plasmids developed to facilitate the construction of Fc-Fusion proteins by fusing a sequence encoding a given protein
to the Fc region of an immunoglobulin. The Fc-fusion proteins are called immunoadhesins when the Fc region is combined with the functional
domain of a binding protein, such as a receptor, ligand or cell-adhesion molecule. The Fc region comprises the CH2 and CH3 domains of the
IgG heavy chain and the hinge region. The hinge serves as a flexible spacer between the two parts of the Fc-Fusion protein, allowing each part
of the molecule to function independently.
➥ Large choice of Fc regions from different species: human, mouse, rat and rabbit
➥ Fc regions without intron (pFUSE-Fc) or with introns (pINFUSE-Fc)
➥ Native Fc regions or engineered Fc regions with altered effector functions MCS
Description
pFUSE-Fc
pFUSE-Fc plasmids are designed for the generation of Fc-fusion proteins. They feature
a large choice of Fc regions from different species, with or without introns, native or
engineered. The gene encoding the Fc region is cloned under the control of a strong
and ubiquitous promoter. A multiple cloning site (MCS) located upstream of the Fc
region facilitates the cloning of the functional domain of a given protein.
pINFUSE-Fc
pINFUSE-Fc is a new family of pFUSE-Fc plasmids featuring genomic Fc regions. The
presence of introns is known to enhance the level of gene expression as splicing is
known to promote rapid and efficient mRNA export.
pFUSE-SEAP-Fc
pFUSE-SEAP-Fc plasmids are control plasmids that express the secreted embryonic
alkaline phosphatase (SEAP) reporter gene fused to each Fc region provided by
InvivoGen.
pFUSE-Fc, pINFUSE-Fc and pFUSE-SEAP-Fc plasmids are selectable with Zeocin in
E. coli and mammalian cells. They can be used for transient or stable transfection.
Features
Secretion of Fc-Fusion Proteins
Two versions of pFUSE-Fc and pINFUSE-Fc exist to allow secretion of the Fc fusion:
• No IL2ss: without the IL2 signal sequence (IL2ss) for the secretion of an Fc-fusion
protein containing the functional domain of a protein that is naturally secreted and
thus contains a native signal sequence.
• With IL2ss contains the IL2 signal sequence (IL2ss) for the generation of Fc-Fusion
proteins derived from proteins that are not naturally secreted.
Ease of Detection
Fc-Fusion proteins can be easily detected in the supernatant of pFUSE-Fc-transfected cells
by SDS-PAGE. Their functional domains can be identified by immunoblotting and ligand
blotting.
Ease of Purification
Fc-Fusion proteins can be easily purified by single-step protein A or protein G affinity
chromatography.
Contents and Storage
All pFUSE-Fc, pINFUSE-Fc and pFUSE-SEAP-Fc plasmids are provided as 20 μg of lyophilized
DNA. Product is shipped at room temperature and should be stored at -20°C. Plasmid is
stable up to one year when properly stored. Each plasmid is provided with 4 pouches of
E. coli Fast-Media ® Zeo (2 TB and 2 Agar, see pages 44-45).
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www.invivogen.com/fc-fusions
MCS
Hinge