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CELL CULTURE & TRANSFECTION 1
Plasmocin - The Mycoplasma Removal Agent
Description
Plasmocin is a well-established antimycoplasma reagent. It contains two
bactericidal components strongly active against mycoplasmas that allow
their elimination in only 2 weeks. The first component acts on the protein
synthesis machinery while the second acts on the DNA replication. These
two specific and separate targets are found only in mycoplasmas and
many other bacteria and are completely absent in eukaryotic cells.
In contrast to other anti-mycoplasma compounds, Plasmocin is active
on both free mycoplasmas and intracellular forms. This advantage is
conferred by one component of Plasmocin which is actively transported
into mammalian cells. It ensures that following treatment with Plasmocin
a cell culture is not reinfected by mycoplasmas released from intracellular
compartments of infected cells.
In all animal cell lines tested to date, even at five times the working
concentration, no apparent adverse effect on cellular metabolism is
observed.
No resistance in liquid cultures of mycoplasmas has ever been identified
in repeated experiments attempting to measure the mutation rate.
Therefore, development of resistant mycoplasma strains is virtually
eliminated.
Plasmocin is also active at low concentrations on a broad range of Gram
positive and Gram negative bacteria that are otherwise resistant to the
mixture of streptomycin and penicillin, and exhibits no toxicity in
eukaryotic cells.
Many cell lines infected by mycoplasmas have been successfully treated
with Plasmocin , including embryonic stem cells, hybridomas and
retrovirus packaging cells.
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www.invivogen.com/mycoplasma
• Plasmocin Treatment
To eliminate mycoplasmas use Plasmocin (ant-mpt) at
25 μg/ml for two weeks in the infected culture.
• Plasmocin Prophylactic
To prevent mycoplasma contamination, use Plasmocin
(ant-mpp) at 2.5 - 5 μg/ml on a regular basis in cell culture.
Plasmocure - The Alternative Mycoplasma Removal Agent
Description
In very rare cases, mycoplasmas resistant to Plasmocin have been
reported. To eradicate these mycoplasmas, InvivoGen has developed a
new antimycoplasma agent called Plasmocure . Plasmocure combines
two antibiotics that act through different mechanisms of action than those
in Plasmocin . A two week treatment with Plasmocure was found
sufficient to completely eliminate the mycoplasmas. A moderate toxicity
can be observed during the course of the treatment but full recovery of
the cell line is expected once mycoplasmas are eliminated.
Plasmocure is a sterile ready-to-use solution. Simply add to mycoplasma
contaminated cell cultures for 2 weeks at the recommended
concentration of 50 μg/ml.
Contents and Storage
Plasmocin is provided as a yellow solution either at a concentration of 25
mg/ml (Plasmocin Treatment) or 2.5 mg/ml (Plasmocin Prophylactic). One
ml Plasmocin Treatment is enough to treat 1 L medium. Plasmocin is
shipped at room temperature. Store at -20°C. Plasmocin is stable 6 months
at 4°C and at least one year at -20°C.
PRODUCT QUANTITY CAT. CODE
Plasmocin Treatment 50 mg (2 x 1 ml) ant-mpt
Plasmocin Prophylactic 25 mg (5 x 2 ml) ant-mpp
Contents and Storage
Plasmocure is provided as a colorless solution at a concentration of 100
mg/ml. One ml Plasmocure is enough to treat 2 L medium. Plasmocure
is shipped at room temperature. Store at -20°C. Plasmocure is stable
one year at 4°C or -20°C.
PRODUCT QUANTITY CAT. CODE
Plasmocure 100 mg (1 x 1 ml) ant-pc