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MAMMALIAN EXPRESSION VECTORS 2
Gene A-List - Human and Murine ORFs
Gene A-List is a collection of high quality, full-length sequenced human and mouse open reading frames (ORFs) available in an expression
vector ready-to-use for protein expression. All ORFs are cloned downstream of a strong and ubiquitous mammalian promoter which makes
these clones suitable for expression and functional studies in various mammalian cell lines.
Features and Benefits
Full-length Sequenced Open Reading Frames
InvivoGen provides a large collection of human and murine genes, fully sequenced. These genes are provided as open reading frames (ORFs) from the ATG
to the Stop codon, excluding introns and untranslated regions. As the ORFs are amplified from cDNA banks, a variant form is sometimes obtained. Most
of the variations have been reported in Genbank. Furthermore for cloning convenience, a neutral amino acid is sometimes added immediately after the
Start codon in order to create a suitable cloning site.
Among the Gene A-List , some genes encode proteins that are naturally secreted. Their sequence include their native signal sequence which is generally
located at the 5' end of the coding sequence. Other genes that code for engineered proteins, such as particular fragments of longer proteins (i.e. angiostatic
proteins) may include the signal sequence of the human IL-2 gene between the ATG and the second codon to allow their secretion.
Suitable for Expression in Mammalian Cells
Each ORF is cloned in a mammalian expression cassette consisting of a potent and ubiquitous composite promoter and the strong SV40 polyadenylation
signal. Each ORF is flanked by a unique restriction site at the 5’ end and 3’ end to facilitate its subcloning into another vector.
The ORFs are supplied in two different plasmid backbones:
• pORF, a plasmid selectable only in E. coli with ampicillin
• pBLAST/pUNO, a plasmid selectable in mammalian cells and in E. coli with blasticidin.
Search online the Gene A-List
You can easily find your ORF of interest in the Gene A-List at www.invivogen.com/ORF.php. You can browse the entire list based on HUGO-approved
gene symbol (and alias) and function. All sequences are available online or can be emailed upon request.
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MAJOR GENE FAMILIES EXAMPLES
Adaptor Genes MyD88, TIRAP, TRAM, TRIF/TICAM1
Angiogenic & Angiostatic Genes ANGPT-1, NRP1, ANGIO, ENDO, FLK1, FLT1
Apoptotic & Anti-Apoptotic Genes BAD, Bcl-XS, BIK, Bcl-XL, Bcl-2, Survivin
Autophagy Genes Atg5, Beclin-1, LC3b
Cellular Matrix Genes Caveolin-1, Decorin
Chemokine & Chemokine Receptor Genes IP-10, LIX, MIG, MIP-1, PF4, SDF1, CCR4, CCR5, CXCR3
Chromatin-Remodeling Genes DNMT1, HDAC1, KAISO, SUV39H1
Connexin Genes Cx26, Cx32, Cx43
Co-Receptor Genes CD14, LBP, MD1, MD2
Co-Stimulatory / CD Genes 4-1BB, B7.1, B7.2, OX40L, CD4, CD8, CD40, CD70
Cytokine & Cytokine Suppressor Genes GM-CSF, IL-2, IL-10, IL-12, LIF, TNF-a, SOCS-1, SOCS-2
Cytolytic Genes Granulysin, Granzyme, Perforin
Cytotoxic / Suicide Genes CD, UPRT, HSV1-TK
DAMP & DAMP Receptor Genes HMGB1, S100A8, S100A9, SAA1, Mincle, RAGE, TREM-1
Growth Factors EGF, FGF, HGF, IGF, TGF-b, VEGF
Hematopoietic Genes EPO, HOXB4, TPO
Immune Receptors FcgR, IL-1R, IL-6R, IL-10R, TNFRSF
Inhibitors of Differentiation Id1, Id2, Id3, Id4
Interferon & Interferon Signaling Genes IFN-a, IFN-b, IFN-g, IRF1, IRF3, IRF5, IRF7
Pattern Recognition Receptors TLR1-13, NOD1, NOD2, NLRP3, MDA-5, RIG-I, Dectin-1
Reprogramming Factors (IPSC) Klf4, Lin28, c-Myc, Nanog, Oct4, Sox2
Signaling Genes AKT, FADD, IPS-1, IRAK, RIP1, STAT, TAK1, TBK1, TRAF6
Signaling Inhibitors A20, Bcl-3, IRAK-M, SIGGIR, SIKE, Tollip
Transcription Factors c-REL, IRF, NF-kB, SMAD, STAT
Tumor Antigens MAGE
Tumor Suppressors HRAS, KRAS, p21, p53, PTEN
www.invivogen.com/orfs