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pORF Description pORF plasmids feature a large collection of native and fusion genes as open reading frames (ORF). The expression of each ORF is under the control of a strong and ubiquitous mammalian promoter. This promoter consists of the elongation factor 1 alpha (EF-1a) core promoter combined to the 5’UTR of either the human T cell leukemia virus (HTLV) type 1 long terminal repeat or the human eukaryotic initiation factor 4g (eIF-4g). These two composite promoters yield similar expression levels. pORF plasmids are selectable in bacteria with ampicillin. The bacterial selection cassette can be replaced upon request with a selection cassette that functions both in bacteria and mammalian cells. This cassette subcloned from the pSELECT can express the resistance gene of your choice. pORF plasmids containing a multiple cloning site (MCS) in place of an ORF were designed to serve as controls and/or cloning vectors. The MCS features commonly used restriction sites to allow convenient insertion of an ORF. Contents and Storage Each pORF plasmid is provided as a lyophilized transformed E. coli strain on a paper disk. Transformed strains are shipped at room temperature and should be stored at -20°C. Lyophilized E. coli cells are stable for at least one year when properly stored. Each pORF is provided with 4 pouches of E. coli Fast-Media ® Amp (2 TB and 2 Agar). pBLAST & pUNO Description pBLAST/pUNO plasmids feature a wide choice of native and fusion genes, mainly involved in innate immunity. The gene of interest is under the control of the same promoter as the pORF plasmids, that is EF- 1a/HTLV or EF-1a/eIF-4g, allowing for strong expression in mammalian cells. pBLAST/pUNO plasmids contain the blasticidin resistance gene (bsr) under the control of a mammalian promoter in tandem with a bacterial promoter. This allows the amplification of the plasmid in E. coli and the selection of stable clones in mammalian cells. Blasticidin is a potent antibiotic that permits the selection of transfectants in only a few days. pBLAST-mcs and pUNO-mcs plamids were designed to serve as control and/or cloning vectors. They carry a multiple cloning site (MCS) that features commonly used restriction sites to allow convenient insertion of an open reading frame. Contents and Storage pBLAST plasmids are provided as 20 μg of lyophilized DNA. pUNO plasmids are provided as lyophilized transformed E. coli strains on a paper disk. Products are shipped at room temperature. Store at -20°C. Plasmids and lyophilized E. coli cells are stable up to one year when properly stored. Each pBLAST and pUNO is provided with 4 pouches of E. coli Fast-Media ® Blas (2 TB and 2 Agar). www.invivogen.com/orfs Related Products Fast-Media ® Amp, page 45 pSELECT, page 32 PRODUCT QTY CAT. CODE pORF- E. coli disk porf- pORF-mcs E. coli disk porf-mcs * Visit www.invivogen.com/ORF.php for complete ordering information Related Products Fast-Media ® Blas, page 45 Blasticidin, page 17 PRODUCT QTY CAT. CODE pBLAST- 20 μg pbla- pBLAST-mcs 20 μg pbla-mcs pUNO- E. coli disk puno- pUNO-mcs E. coli disk puno-mcs * Visit www.invivogen.com/ORF.php for complete ordering information 37 MAMMALIAN EXPRESSION VECTORS 2
MAMMALIAN EXPRESSION VECTORS 2 PromTest - Ten Promoters to Choose From Promoters regulate differently gene expression depending on the cellular context. To help you determine easily and rapidly the best promoter for your cell line, InvivoGen offers PromTest , a collection of ten ubiquitous promoters driving the GFP reporter gene. ➥ Ten ready-to-use promoters with different levels of expression ➥ Drive GFP expression for convenient monitoring of promoter strength ➥ Cost-effective Description PromTest is a collection of ten ubiquitous composite promoters provided in the pDRIVE5-GFP plasmid. These composite promoters were generated by assembling enhancers, core promoters and 5’UTRs of different origins. The activity of each combination depends on the cellular context (see graphs). pDRIVE5-GFP features a GFP reporter gene for convenient monitoring of promoter activity. The strength of each promoter can be assessed qualitatively by fluorescence microscopy and quantitatively using a fluorometer or flow cytometry. pDRIVE5-GFP plasmids are selectable in E. coli with Zeocin . PromTest contains 5 μg of each plasmid, enough for multiple transfections using your favorite reagent or technique. pDRIVE5-GFP plasmids can be amplified in any common E. coli laboratory strains. They are also available individually as 20 μg high-quality endotoxin-free DNA. Once you have determined the best promoter for your cell line and application, you can either replace the GFP gene with your gene of interest, or subclone the promoter into another plasmid with mammalian selection, such as InvivoGen’s pSELECT. Evaluation of PromTest in different cell lines: HeLa (human cervical cancer) and CHO (chinese hamster ovary) cells were transiently transfected with each of the 10 pDRIVE5- GFP plasmids of PromTest using LyoVec (cat. code: lyec-12). The strength of the various promoters was analyzed by flow-cytometry 48h after transfection. Abbreviations: chEF1, chimpanzee elongation factor 1 alpha; hAlda, human aldolase A; hCMV, human cytomegalovirus; hEF1, human elongation factor 1 alpha; hFerL, human ferritin light chain; HTLV, human T lymphocyte virus; mCMV, mouse cytomegalovirus; mTyr, mouse tyrosinase; SV40, simian virus 40. Contents and Storage PromTest contains 10 plasmids, each provided as 5 μg lyophilized DNA Plasmids can also be purchased individually as 20 μg high-quality endotoxin-free DNA. Plasmids are shipped at room temperature. Store at -20°C. Plasmids are stable up to one year when properly stored. 38 Plasmid Enhancer Core Promoter pDRIVE5-GFP-1 pDRIVE5-GFP-2 pDRIVE5-GFP-3 pDRIVE5-GFP-4 pDRIVE5-GFP-5 pDRIVE5-GFP-6 pDRIVE5-GFP-7 pDRIVE5-GFP-8 pDRIVE5-GFP-9 pDRIVE5-GFP-1 0 hCMV mCMV SV40 mTyr hCMV SV40 hCMV mCMV SV40 hAldA hEF1 hEF1 hEF1 hEF1 hCMV hCMV hFerL hFerL hFerL hFerL 5’ UTR HTLV HTLV HTLV HTLV HTLV HTLV chEF1 chEF1 chEF1 chEF1 www.invivogen.com/promoters PRODUCT QTY CAT. CODE PromTest 10 x 5 μg prom-test pDRIVE5-GFP-n 20 μg pdv5-gfp
Page 2 and 3: INNOVATION WITHIN REACH InvivoGen
Page 4 and 5: CONTENTS AT A GLANCE 1 CELL CULTURE
Page 6 and 7: 4 CYTOKINE SIGNALING TABLE of CONTE
Page 8 and 9: Mycoplasma Detection & Elimination
Page 10 and 11: PlasmoTest Contents - HEK-Blue -2
Page 12 and 13: Primocin - The Antimicrobial Shiel
Page 14 and 15: SEAP Reporter Gene System Secreted
Page 16 and 17: HEK-Blue Detection - Real-Time Det
Page 18 and 19: Blasticidin S Description Blasticid
Page 20 and 21: LyoVec New formulation LyoVec is
Page 22 and 23: Poly-Arginine-HA Tagged Reprogrammi
Page 24 and 25: LENTI-Smart - Lentiviral Vector Pr
Page 26 and 27: Lentiviral Vector Production and Ce
Page 28 and 29: Antibody generation using pFUSE-CHI
Page 30 and 31: Engineering Fc regions for altered
Page 32 and 33: pBROAD & pWHERE - Optimized Vector
Page 34 and 35: pSELECT-Tag - Expression of a GFP-,
Page 36 and 37: pVIVO - Expression of Two Genes of
Page 40 and 41: Prom A-List - Human and Rodent Pro
Page 42 and 43: PROMOTER GENE TISSUE DISTRIBUTION S
Page 44 and 45: ChemiComp E. coli ChemiComp E. coli
Page 46 and 47: Fast-Media ® Preparation 1. Empty
Page 48 and 49: RIG-I-like Receptors 54 RLR & RLR-R
Page 50 and 51: Toll-Like Receptors Toll-Like Recep
Page 52 and 53: www.invivogen.com/innate-immunity 5
Page 54 and 55: IPAF and NAIP5 IPAF (NLRC4, CLAN/CA
Page 56 and 57: C-Type Lectin Receptors C-type lect
Page 58 and 59: pUNO pUNO-HA Toll-Like Receptors (T
Page 60 and 61: GENE NAME/ALIASES DESCRIPTION CAT.
Page 62 and 63: pDUO - Gene Associations Features a
Page 64 and 65: pZERO-TLR & pDeNy - Dominant Negati
Page 66 and 67: 293/TLR & 293/NOD Clones 293/TLR Cl
Page 68 and 69: Response of HEK-Blue hTLR2 cells t
Page 70 and 71: Ramos-Blue Cells - NF-kB/AP-1 Repo
Page 72 and 73: MEF Cells C3H/TLR4mut & C3H/WT MEFs
Page 74 and 75: Antibodies for Detection ANTIBODY D
Page 76 and 77: - NF-kB binding site: Nuclear facto
Page 78 and 79: PRODUCT ORIGIN/DESCRIPTION TLR2 Ago
Page 80 and 81: PRODUCT ORIGIN/DESCRIPTION TLR9 Ago
Page 82 and 83: PRODUCT ORIGIN/DESCRIPTION Dectin-1
Page 84 and 85: LPS-PG (Porphyromonas gingivalis) R
Page 86 and 87: • FLA-ST ultrapure was purified b
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1. Zhao H. et al., 2004. Contributi
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1. Hornung V. et al., 2006. 5'-Trip
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TLR/NOD Ligand Screening InvivoGen
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Signal Transduction Inhibitors Invi
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Pepinh-MYD - MyD88 inhibitory pepti
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1. Martinon F,. et al., 2002. The i
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Nigericin - NLRP3 Inflammasome Indu
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Autophagy is a pathway by which cyt
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4 CYTOKINE SIGNALING Cytokine Genes
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Blue Cytokine Reporter Cells Blue
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HEK-Blue IFN-a/b Cells - Human Typ
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HEK-Blue IL-6 Cells - IL6 Reporter
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HEK-Blue IL-1b Cells - IL-1b Repor
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HEK-Blue IL-33/IL-1b Cells - IL-33
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HEK-Blue CD40L Cells - CD40L Repor
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5 ANTIBODIES & VACCINATION Antibodi
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Antibody generation using pFUSE-CHI
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Immunoglobulin A The mucosal surfac
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IgA Detection and Quantification In
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IgG Antibodies InvivoGen offers a s
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Mediators? 1. Leroux-Roels G., 2010
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Poly(I:C) Synthetic double-stranded
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pVAC - Induction of Neutralizing An
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CpG-Free DNA CpGs and the Immune Re
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pCpGfree-basic & pCpGfree-promoter
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pCpGfree-siRNA & pCpGfree-siRNADUO
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Custom-Made CpG-Free Genes InvivoGe
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RNA INTERFERENCE 7 psiRNA System I
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RNA INTERFERENCE 7 psiRNA-h7SK - 7S
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RNA INTERFERENCE 7 psiTEST System
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RNA INTERFERENCE 7 Ready-Made psiRN
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RNA INTERFERENCE 7 Custom-Made psiR
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INHIBITORS 8 Inhibitors PRODUCT DES
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INHIBITORS 8 Pattern Recognition Re
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INHIBITORS 8 Triptolide - NF-kB Inh
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INHIBITORS 8 Heat Shock Protein Inh
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9 CUSTOM SERVICES TLR/NOD Ligand Sc
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CUSTOM SERVICES 9 Custom Cloning :
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CUSTOM SERVICES 9 Prices 164 TERMS
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166 PRODUCT (QUANTITY) CAT. CODE PA
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A 17-AAG 17-AAGH2 17-AEP-GA 2-Amino
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- ODN 4084-F - ODN INH-1 - ODN INH-
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ARGENTINA Genbiotech S.R.L. Tel: +5
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